和田茴香籽总黄酮提取及体内抗氧化活性研究

Study on Extraction of Total Flavonoids from Hetian Fennel Seeds and Their Antioxidant Activity in Vivo

随着科技的发展,黄酮提取相关研究越来越多。和田茴香广泛种植于我国西南地区,是一种典型的调味料,茴香籽是茴香种子,产量较高,除了用作种子之外,多余的茴香籽常被丢弃,造成大量资源浪费。该研究基于此,利用单因素试验和正交试验,对和田茴香籽总黄酮加工工艺进行研究和优化,研究结果表明,和田茴香籽总黄酮最佳加工工艺为乙醇浓度80%、提取时间2.5 h、料液比例1∶40、提取温度70℃,此加工条件下,和田茴香籽总黄酮得率为1.531%。另外,对和田茴香籽总黄酮抗氧化活性进行研究,结果表明,饲喂和田茴香籽总黄酮的小鼠,血清和肝匀浆中的SOD值相比空白对照组均提升,MDA值均降低,说明一定量的和田茴香籽总黄酮能够提高SOD值,降低MDA值,从而达到减少小鼠体内自由基堆积和清除氧化物的作用。

With the development of science and technology,there are more and more researches on the extraction of flavonoids.Hetian fennel is widely planted in the southwest of China and is a typical seasoning.The yield of fennel seeds is high.In addition to being used as seeds,excess fennel seeds are often discarded,resulting in a large amount of resource waste.Based on this,in this study,single factor test and orthogonal test are used to study and optimize the processing technology of total flavonoids of Hetian fennel seeds.The research results show that the optimal processing technology of total flavonoids of Hetian fennel seeds is ethanol concentration of 80%,extraction time of 2.5 h,solid-liquid ratio of 1∶40 and extraction temperature of 70℃.Under these processing conditions,the yield of total flavonoids of Hetian fennel seeds is 1.531%.In addition,the antioxidant activity of total flavonoids of Hetian fennel seeds is studied.The results show that the SOD values in serum and liver homogenate of the mice fed with the total flavonoids of Hetian fennel seeds increase compared with the blank control group,and the MDA values decrease,indicating that a certain amount of total flavonoids of Hetian fennel seeds can increase the SOD values and reduce the MDA values,so as to achieve the effect of reducing free radical accumulation and scavenging oxides in mice.