荞麦槲皮素改善脂代谢异常肝原代细胞的作用机制

Mechanism of Buckwheat Quercetin in Alleviating Abnormal Lipid Metabolism in Primary Hepatocytes

为探讨荞麦槲皮素干预肝脂代谢紊乱作用机制,该研究以棕榈酸刺激小鼠肝原代细胞为模型,利用转录组学技术建立肝原代细胞基因库,探究荞麦槲皮素在改善脂代谢异常肝原代细胞中的生物学作用。结果表明,棕榈酸刺激和槲皮素干预在基因表达上差异显著,槲皮素能够减弱缺氧诱导因子介导的内质网应激进而减缓脂肪的脂解作用,通过阻止游离脂肪酸的运输减少其向肝细胞的流入,从而提高胰岛素敏感性以抑制肝脏葡萄糖的产生路径。该研究揭示荞麦槲皮素通过减缓脂解作用改善脂代谢异常的肝原代细胞相关机制。

The mechanism of buckwheat quercetin in the intervention of hepatic lipid metabolism disorders was studied.With the palmitic acid-stimulated mouse primary hepatocytes as a model,transcriptomics was employed to establish a gene pool of primary hepatocytes.The biological role of buckwheat quercetin in alleviating the abnormal lipid metabolism of primary hepatocytes was explored.The results showed that palmitic acid stimulation and quercetin intervention groups differed significantly in gene expression.Quercetin attenuated hypoxia-inducible factor-mediated endoplasmic reticulum stress and thus slowed down lipolysis.Furthermore,it reduced the influx of free fatty acids into hepatocytes by blocking their transport,and thus improved insulin sensitivity to inhibit the glucose production pathway in the liver.The findings revealed that buckwheat quercetin ameliorated the abnormal lipid metabolism in primary hepatocytes by slowing down lipolysis.