贻贝多糖对胰岛素抵抗HepG2细胞糖代谢的影响

Effects of Mussel Polysaccharides on Glucose Metabolism in Insulin-resistant HepG2 Cells

为了探究贻贝多糖对胰岛素抵抗(Insulin resistance,IR)HepG2细胞糖代谢影响的潜在分子机制,以贻贝多糖(Mussel polysaccharides,MP)为研究材料,采用CCK8法检测HepG2细胞的增殖情况,同时筛选不同浓度胰岛素构建细胞胰岛素抵抗模型,检测MP对胰岛素抵抗HepG2细胞葡萄糖消耗的影响。结果显示:当胰岛素浓度处于10-6 mol/L时,HepG2细胞的葡萄糖含量最高,对葡萄糖的消耗能力最弱,是构建胰岛素抵抗模型的最佳浓度。此外,MP(100~1000μg/mL)对HepG2细胞无毒性,能促进细胞增殖。与IR-HepG2细胞相比,200、400、600μg/mL的MP能明显提升糖原含量,分别为17.20%、22.95%和32.50%。同时,高剂量MP能显著上调PI3K和GLUT2的相对基因表达量,并能降低GSK-3β的表达量。为后续MP降血糖提供实验基础,同时有助于促进MP的开发利用。

This study aimed to evaluate the effects of mussel polysaccharides on glucose metabolism in insulin resistance HepG2 cells.Using the mussel polysaccharide(MP)as the research material,the CCK8 method was used to detect the proliferation of HepG2 cells,and different concentrations of insulin were screened to construct a cellular insulin resistance model to detect the effect of mussel polysaccharide on glucose consumption of insulin-resistant HepG2 cells.The results showed that when the insulin concentration was 10−6 mol/L,the glucose content of HepG2 cells was the highest and the ability to consume glucose was the weakest,and it was the best concentration for constructing the insulin resistance model.MP(100-1000μg/mL)were non-toxic to HepG2 cells,could promote cell proliferation.Compared with IR-HepG2 cells,200,400 and 600μg/mL of MP significantly elevated glycogen content by 17.20%,22.95%and 32.50%,respectively.Meanwhile,high-dose MP significantly up-regulated the relative gene expression of PI3K and GLUT2,decreased the expression of GSK-3β.It provides an experimental basis for the subsequent hypoglycemia of MP,and also helps to promote the exploitation and potential application of MP.