五种豆科植物WRKY基因家族全基因组鉴定及表达分析

Genome-wide Identification and Expression Analysis of WRKY Gene Family in Five Legumes

为了增强对豆科植物WRKY基因的多样性和进化上的认识,探索WRKY转录因子家族成员的功能和在育种中的应用。在本研究中,利用生物信息学方法对5种豆科植物大豆、鹰嘴豆、菜豆、蒺藜苜蓿及百脉根的WRKY基因进行了染色体定位、系统进化、保守基序、基因结构和基因复制等方面的分析。从上述5种豆科植物中分别鉴定出185、61、90、108和83个WRKY基因。将5种豆科植物WRKY蛋白分为三类和五个亚类,同一亚类具有相似的蛋白结构和基因结构。5种豆科植物的WRKY基因家族成员中存在基因复制事件,并且在各个组织中有明显差异表达。WRKY基因在不同组织中的表达模式表明,它们可能在豆科植物的生长发育中发挥重要作用。

In order to enhance understanding of the diversity and evolution of WRKY genes in leguminous plants,and to explore the functions of WRKY transcription factor family members and their applications in breeding,in this study,we analyzed the classification,basic physicochemical properties,evolutionary relationship,gene structure,chromosome location,conserved motifs,promoter elements,gene collinearity,expression in five legumes(Glycine max,Cicer arietinum,Phaseolus vulgaris,Medicago truncatula,Lotus japonicus)by using bioinformatics.A total of 185,61,90,108 and 83 WRKY genes were identified,respectively.WRKY protein were identified,and the classification,basic physicochemical properties,evolutionary relationship,gene structure,chromosome location,conserved motifs,promoter elements,gene collinearity,expression analysis were systematically analyzed.The WRKY proteins in all five species were divided into three classes and five subclasses.The WRKY proteins derived from the same evolutionary clade were found to have similar genes and protein structures.There are gene replication events in members of WRKY gene family of the five leguminous plants,and there are significant differences in expression in each tissue.The expression patterns of WRKY genes in different tissues indicate that they may play an important role in the growth and development of leguminous plants.