大豆GmHMGR基因响应外源激素及非生物胁迫功能研究

Functional Analysis of Soybean Gene GmHMGR Responding to Exogenous Hormones and Abiotic Stresses

GmHMGR基因是大豆甲羟戊酸途径的关键酶基因之一,在大豆逆境胁迫调控方面具有重要的作用。探究GmHMGR基因在逆境胁迫中的功能,为大豆抗氧化、抗盐机制解析和分子育种提供依据。采用实时荧光定量PCR、酵母转化、拟南芥遗传转化等技术,分析GmHMGR基因在响应外源激素和非生物胁迫中发挥的功能。结果显示,GmHMGR基因对PEG6000、NaCl、H2O2胁迫以及MeJA、ABA外源激素处理均有响应;过量表达GmHMGR1、GmHMGR3、GmHMGR5、GmHMGR6和GmHMGR7可以提高酵母的抗盐、抗氧化能力;过量表达GmHMGR4与GmHMGR6的拟南芥上调了萜类代谢相关酶基因(AtDXR、AtSQS1、AtSQS2与AtCAS)的表达水平,改善了拟南芥在氧化及盐胁迫下的表型。GmHMGR基因可能参与大豆抗氧化、抗盐胁迫的应答。

GmHMGR gene is one of the key enzyme genes in the mevalonate pathway of soybean(Glycine max L.),and plays an important role in the stress regulation.Exploring the function of the GmHMGR gene in soybean stress resistance will serve as a foundation for the analysis of soybean antioxidant,salt resistance mechanisms,and molecular breeding.The function of the GmHMGR gene under abiotic stress and hormone treatments was analyzed using real-time quantitative PCR,yeast transformation,and A.thaliana genetic transformation.The results showed that the GmHMGR gene responded to PEG6000,NaCl,H2O2,MeJA and ABA treatments.Overexpressing GmHMGR1,GmHMGR3,GmHMGR5,GmHMGR6 and GmHMGR7 genes improved yeast's salt resistance and antioxidant capacity.Overexpressing GmHMGR4 and GmHMGR6 genes in A.thaliana upregulated the expression levels of terpenoids metabolism-related enzymes genes(including AtDXR,AtSQS1,AtSQS2 and AtCAS),and improved its resistance capacity under oxidative and salt stress.GmHMGR gene may be involved in response to oxidative and salt stresses in soybean.