基于Super-GBS的高粱株高和节间数QTL定位

QTL Mapping of Sorghum Plant Height and Internode Numbers Based on Super-GBS Technique

株高及相关性状是影响高粱株型和产量的关键因素,定位影响这些性状的主效QTL,为高粱的分子遗传改良提供依据。以美国高粱品种BTx623和贵州酒用高粱品种红缨子杂交构建的包含205个家系的重组自交系(RIL)群体为材料,在5个环境条件下调查株高和节间数。利用Super-GBS技术进行基因分型,建立SNP标记遗传图谱,采用完备区间作图法(ICIM)开展QTL定位。结果表明,在第1、3、4、8、9染色体上一共检测到18个QTL,其中,与株高和节间数相关的QTL分别为7和11个。有10个主效QTL在多个环境中或2个性状中被重复检测到,其中q PH9.1与已知的株高基因Dw1一致,而q PH1.2、qPH3.2、qIN3.2以及q IN8.1所在区间内包含了4个与影响水稻株高或节间伸长的基因同源。

Plant height and its relative traits are key factors that affect plant type and yield of sorghum.Mapping major QTL for these traits may provide a basis for genetic improvement in sorghum.In this study,a RI population including 205 lines obtained from a cross between American cultivar BTx623 and Guizhou liquor-brewing cultivar Hongyingzi was used to investigate plant height and internode numbers under five environments.The genetic map was constructed based on genotype data from Super-GBS technology,and major QTLs were identified by inclusive composite interval mapping(ICIM).Totally,eighteen major QTLs were detected on chromosome 1,3,4,8,and 9,of which 7 and 11 QTLs were related to plant height and internode numbers,respectively.Ten major QTLs were repeatedly detected in multiple environments or two traits,of which qPH9.1 was consistent with the known plant high gene Dw1,while qPH1.2,qPH3.2,qIN3.2 and qIN8.1 were colocalized with four homologous genes involving in plant height or internode elongation in rice.