高效反硝化聚磷菌的筛选及其脱氮除磷条件和性能研究

Screening of an Efficient Denitrifying Phosphorus-accumulating Bacterium and Its Denitrification and Phosphorus Removal

筛选高效反硝化聚磷菌,探究其脱氮除磷条件及对模拟废水的处理性能。纯培养技术分离反硝化聚磷菌;基于16S rRNA基因序列分析和生理生化特征,完成菌株D4的初步鉴定;通过单因素和响应面法优化脱氮除磷条件,并应用于食品模拟废水的生物脱氮除磷。反硝化聚磷细菌D4为戈登氏菌(Gordonia sp.),脱氮除磷的最佳条件为乙酸钠(3.32 g/L)作碳源、接种量5%、初始磷含量18.61 mg/L、31.3℃、pH 7.9,该条件下菌株D4对总磷、硝氮和氨氮的去除量分别为14.18、39.67和69.71 mg/L,相应去除率为84.50%、97.67%和96.22%;应用于不同模拟废水的结果显示,菌株D4对多种废水均具有良好的脱氮除磷效果,其中对豆制品废水中的氮磷去除效果最好,脱氮量和除磷量分别达101.58 mg/L和10.40 mg/L。戈登氏菌D4具有较好的反硝化聚磷能力,是豆制品废水等高氮高磷类食品加工废水生物脱氮除磷的良好菌种资源。

The objective is to explore the denitrification and phosphorus removal conditions and performance of a efficient denitrifying phosphorus-accumulating bacterium,and its treatment effect on simulated wastewater.Denitrifying phosphorus-accumulating strain D4 was isolated by pure culture technology.Preliminary identification of the strain was completed based on 16S rRNA gene sequence analysis and physiological and biochemical characteristics,and the denitrification and dephosphorization conditions were optimized by single factor and response surface method,so as to achieve efficient biological denitrification and dephosphorization of mimic food wastewater.As results,denitrifying phosphorus-accumulating bacterium D4 was Gordonia sp..The optimal conditions for nitrogen and phosphorus removal were sodium acetate(3.32 g/L)as carbon source,5%inoculum,initial phosphorus content of 18.61 mg/L,31.3℃,and pH 7.9.Under these conditions,the removed amounts of total phosphorus,nitrate nitrogen and ammonia nitrogen by strain D4 were 14.18,39.67 and 69.71 mg/L,respectively;and the corresponding removal rates were 84.50%,97.67% and 96.22%.Results of treatment to different simulated wastewater showed that strain D4 had the best nitrogen and phosphorus removal effects in soybean product wastewater,with the removed amount of nitrogen and phosphorus being 101.58 mg/L and 10.40 mg/L,respectively.In conclusion,strain D4 has promising denitrification and phosphorus-accumulating ability and is a good strain resource for biological denitrification and phosphorus removal of food processing wastewater with high nitrogen and phosphorus such as soybean product wastewater.