健脾益肾方对痛风性肾损害患者尿液微RNA表达变化的研究

Study on the changes of urinary microRNA expression of Jianpi Yishen decoction in patients with gout renal damage

目的分析健脾益肾方干预前后痛风性肾损害患者尿液微RNA(microRNA,miRNA)的表达变化。方法通过高通量测序法检测早期痛风性肾损害患者干预前后尿液外泌体源性miRNA的表达谱,并比较表达变化情况。结果早期痛风性肾损害患者与正常人群的尿液外泌体源性miRNA有明显差异;早期痛风性肾损害患者健脾益肾方中药干预后,19个可作为生物标记物的miRNA中有5个平均表达值上调,14个平均表达值下调。结论健脾益肾方对痛风性肾损害患者的miRNA表达有一定调节作用,差异表达的miRNA可能参与肾损害的发生发展。

Objective To analyze the changes of microRNA(miRNA)expression in urine of patients with gout renal damage before and after the intervention of Jianpi Yishen decoction.Methods The expression profiles of urine exosome-derived miRNAs were detected by high-throughput sequencing before and after intervention in patients with early gout renal damage,and the expression changes were compared.Results Urine exosome-derived miRNAs were significantly different between the patients with early gout renal damage and the normal population.After the intervention of Jianpi Yishhen decoction,the expression of 19 miRNAs that could be used as biomarkers were up-regulated in 5 and down-regulated in 14.Conclusion Jianpi Yishen decoction can regulate the expression of miRNA in patients with gout renal damage to some extent,and differentially expressed miRNA may be involved in the occurrence and development of renal damage.