高效液相色谱法测定新生儿咖啡因血药浓度及临床应用

Determination of Caffeine Blood Concentration in Neonates by High Performance Liquid Chromatography and Its Clinical Application

目的:建立测定新生儿血清中咖啡因血药浓度的高效液相色谱法,并对使用咖啡因治疗原发性呼吸暂停新生儿的血药浓度进行测定。方法:色谱柱为ZORBAX Extend C18柱,流动相为乙腈-水(19∶81,V/V),柱温25℃,流速1 mL/min,检测波长273 nm。结果:咖啡因血药浓度在1.0~160.0μg/mL范围内呈良好的线性关系(r=0.998 9),平均回收率98.40%~102.40%,日内与日间相对标准差(RSD)≤9.63%,检测10例患儿给药7 d后咖啡因血药浓度为(20.53±11.12)μg/mL。结论:建立的高效液相色谱法准确、快速,经验证适用于新生儿血清中咖啡因血药浓度测定。

Objective:To establish the high performance liquid chromatography(HPLC)method for the determination of caffeine blood concentration in neonates,and to determine the concentration of caffeine in neonates with primary apnea.Methods:The determination was performed on ZORBAX Extend C18 column with mobile phase acetonitrile-water(19∶81,V/V)at the column temperature of 25℃and flow rate of 1 mL/min.The detection wavelength was 273 nm.Results:Caffeine blood concentration showed good linearity(r=0.998,9)in the range of 1.0 to 160.0μg/mL,with mean recoveries of 98.40%to 102.40%.Inter-day and intra-day relative standard deviation(RSD)was≤9.63%.The average caffeine blood concentration in 10 children was(20.53±11.12)μg/mL after 7 d of administration.Conclusion:The established high performance liquid chromatography method is accurate,rapid and validated for the determination of caffeine blood concentration in neonates.