摘要
用于实时成像活细胞DNA的细胞核探针是非常罕见的。通过Ru(N^N)3、Ir(C^N)2(N^N)、Re(CO)3Cl(N^N)和Pt(C^N)(N^N)的结合,生成了4种异核双金属配合物:2种不同的功能金属位点被锚定在线性双齿螯和配体(N^N)-(N^N)的尾端,形成不对称哑铃状的双金属分子M1-M2,即Ru-Re、Ru-Pt、Ir-Re和Ir-Pt。Ru-Re和Ru-Pt的红色发射探针具有较大的斯托克斯位移、优异的核膜穿透能力、良好光稳定性的DNA结合能力。此外,Ru-Re和Ru-Pt探针的DNA成像可与专门用于活细胞细胞核DNA染色的Hoechst 33342商用染料相媲美,而Ir-Re和Ir-Pt探针直接靶向线粒体。不同探针的发光特性和选择性胞内定位高度依赖于在异核双金属配合物中的金属物种。
Nucleus probes for real-time imaging of DNA in live cells are very rare.Herein,the combined use of Ru(N^N)_(3),Ir(C^N)2(N^N),Re(CO)_(3)Cl(N^N),and Pt(C^N)(N^N)produced four heterobimetallic complexes.Two differ-ent functional metal sites are arranged at both ends of linear bis-chelating linkers(N^N)-(N^N),leading to asymmet-ric dumbbell-like molecules M1-M2,namely,Ru‑Re,Ru‑Pt,Ir‑Re,and Ir‑Pt.The red emissive probes of Ru‑Re and Ru‑Pt offer the benefits of large Stokes shift,excellent nucleus membrane penetration,and DNA binding ability with good photostability.Moreover,DNA imaging of Ru‑Re and Ru‑Pt probes was comparable to the commercial dye of Hoechst 33342 which is specially used for staining DNA in the nucleus of live cells.However,the Ir-based probes of Ir‑Re and Ir‑Pt directly targeted mitochondria.The emission properties and selective intracellular local-izations of different probes are highly dependent on the metal species used in the heterobimetallic complexes.
作者
王莹
薛瑞
黄胜利
杨国昱
WANG Ying;XUE Rui;HUANG Sheng-Li;YANG Guo-Yu(MOE Key Laboratory of Cluster Science,School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 100081,China)
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2023年第8期1463-1470,共8页
Chinese Journal of Inorganic Chemistry
基金
国家自然科学基金(No.21971011,21831001)资助。