CDKN2A基因突变与肺癌患者预后的关系

Effects of CDKN2A Gene Mutations on Lung Cancer and Their Relationship with Prognosis

目的 探讨细胞周期蛋白依赖性激酶抑制基因(CDKN2A)突变与肺癌患者预后的关系。方法 选取79例肺癌患者,所有患者均于术前采集癌变组织及癌旁组织样本,开展CDKN2A免疫组织化学检测、CDKN2A基因突变检测,并随访3年。分析CDKN2A基因在肺癌组织内突变情况及与其临床病理特征、预后的关系,并进行COX回归分析。结果 肺癌组织CDKN2A基因高表达率为13.92%(12/79),低于癌旁组织的84.81%(68/79),基因突变率37.97%(30/79),较癌旁组织的7.59%(6/79)高,有统计学差异(P<0.05);基因突变患者复发转移与基因野生患者相比,有统计学差异(P<0.05);基因突变患者3年生存率为46.67%(14/30),较基因野生患者[77.55%(38/49)]低,中位生存时间为(22.72±3.13)个月,短于基因野生患者[(34.25±4.11)]个月,有统计学差异(P<0.05);COX回归分析显示,肿瘤大小、CDKN2A基因突变、复发转移为影响肺癌术后生存期的独立危险因素(P<0.05)。结论 CDKN2A基因突变与肺癌患者预后关系密切,可直接影响肺癌患者术后生存率。

Objective To investigate the relationship between mutations in the cyclin dependent kinase inhibitor gene(CDKN2A) and the prognosis of lung cancer patients.Methods A total of 79 patients with lung cancer were selected.Samples of cancerous and paracancerous tissues were collected from all patients before surgery,and CDKN2A immunohistochemical testing and CDKN2A gene mutation testing were performed.All patients were followed up for 3 years.To analyze the mutation of CDKN2A gene in lung cancer tissue and its relationship with clinicopathological characteristics and prognosis,and to conduct COX regression analysis.Results The high expression rate of CDKN2A gene in lung cancer tissue was 13.92%(12/79),lower than 84.81%(68/79) in paracancerous tissue,and the gene mutation rate was 37.97%(30/79),higher than 7.59%(6/79) in paracancerous tissue,with a statistically significant difference(P<0.05);The recurrence and metastasis of patients with gene mutations were significantly different from those with wild genes(P<0.05);The 3-year survival rate of gene mutation patients was 46.67%(14/30),which was lower than that of gene wild patients(77.55%(38/49)).The median survival time was(22.72±3.13) months,which was shorter than that of gene wild patients(34.25±4.11),with a statistically significant difference(P<0.05);COX regression analysis showed that tumor size,CDKN2A gene mutation,and recurrence and metastasis were independent risk factors affecting postoperative survival of lung cancer(P<0.05).Conclusion CDKN2A gene mutations are closely related to the prognosis of lung cancer patients,and can directly affect the survival rate of lung cancer patients after surgery.