基于网络药理学对三才封髓丹组方治疗复发性口腔溃疡的作用机制研究

Study on the Mechanism of Action of Sancai Fengsui Dan in the Treatment of Recurrent Aphthous Ulcer Based on Network Pharmacology

目的:研究三才封髓丹治疗复发性口腔溃疡的网络药理学作用机制。方法:利用TCMSP、BATMAN数据库预测和筛选出三才封髓丹治疗复发性口腔溃疡的潜在靶点,采用CTD和DisGeNET数据库对口腔溃疡相关基因和靶标筛选,并与前面数据库筛选的靶标整合,缩小范围。基于Cytoscape插件对获得的靶点蛋白进行GO富集分析和KEGG通路注释,绘制蛋白相互作用网络和“活性成分-靶点-信号通路”网络图确定组方的作用机制。结果:组方中71个活性成分被筛选出。通过交叉验证55个靶蛋白显著富集28条GO功能术语中,最后划分为8个类别功能。靶蛋白主要通过JAK-STAT信号通路、细胞因子-受体相互作用、PI3K-Akt信号通路等通路作用。基于草药-成分-靶蛋白-通路构建网络药理学网络,最终构建网络共122个节点,363条关系对,其中化学成分节点27个,靶蛋白节点73个,通路相关靶蛋白节点35个,通路节点17个。结论:本研究结果初步预测了三才封髓丹治疗复发性口腔溃疡的主要活性成分、靶点和相关通路,为进一步深入揭示其作用机制奠定了良好基础。

Objective:To study the mechanism of network pharmacology of Sancai Fengsui Dan in the treatment of recurrent aphthous ulcer.Method:TCMSP and BATMAN databases were used to predict and screen out potential targets of Sancai Fengsui Dan in the treatment of recurrent aphthous ulcer.CTD and DisGeNET databases were used to screen oral ulcer-related genes and targets,and integrated with the targets screened in the previous databases to narrow down the scope.GO enrichment analysis and KEGG pathway annotation were carried out on the obtained target proteins based on Cytoscape plug-in.Protein interaction network and"bioactive componenttarget-signaling pathway"network map were drawn to determine the mechanism of action of the formula.Result:71 active ingredients were screened.Through cross-verification,55 target proteins were significantly enriched in 28 GO functional terms,which were finally divided into 8 functional categories.The target protein mainly acts through JAKSTAT signaling pathway,cytokine-receptor interaction,PI3K-Akt signaling pathway and other pathways.A network pharmacological network was constructed based on the herb-composition-target protein-pathway.The final network consisted of 122 nodes and 363 relationship pairs,including 27 chemical composition nodes,73 target protein nodes,35 pathway-related target protein nodes,and 17 pathway nodes.Conclusion:The results of this study preliminarily predicted the main bioactive component,targets and related pathways of Sancai Fengsui Dan in the treatment of recurrent aphthous ulcer,and laid a good foundation for further revealing its mechanism of action.