摘要
猪流行性腹泻(porcine epidemic diarrhea,PED)是一种可引起仔猪高致死率的传染性疾病,尽管已有灭活疫苗和减毒活疫苗上市,但由于病毒变异频繁导致保护效力欠佳,发病率依然居高不下。本研究首次制备了PED mRNA候选疫苗,并在小鼠和妊娠母猪上评价了其免疫原性,证明了基于病毒受体结合区异源二聚体的mRNA候选疫苗具有良好的免疫原性,可在小鼠上高效诱导体液和细胞免疫应答,单次免疫血清中和抗体滴度达到1:300以上,并在妊娠母猪上诱导了与灭活疫苗相近的中和抗体水平,实现了100%抗体转阳。本研究为PED mRNA疫苗的进一步产业化奠定了基础。
Porcine epidemic diarrhea(PED)is a highly contagious disease that causes high mortality in suckling piglets.Although several licensed inactivated and live attenuated vaccines were widely used,the infection rate remains high due to unsatisfactory protective efficacy.In this study,mRNA vaccine candidates against PED were prepared,and their immunogenicity was evaluated in mice and pregnant sows.The mRNA PED vaccine based on heterodimer of viral receptor binding region(RBD)showed good immunogenicity.It elicited robust humoral and cellular immune responses in mice,and the neutralizing antibody titer reached 1:300 after a single vaccination.Furthermore,it induced neutralizing antibody level similar to that of the inactivated vaccine in pregnant sows.This study developed a new design of PED vaccine based on the mRNA-RBD strategy and demonstrated the potential for clinical application.
作者
杨利敏
王俊虹
徐明国
王虎
张晓娟
刘文军
陈创夫
YANG Limin;WANG Junhong;XU Mingguo;WANG Hu;ZHANG Xiaojuan;LIU Wenjun;CHEN Chuangfu(Key Laboratory of Zoonoses,College of Animal Science and Technology,Shihezi University,Shihezi 832000,Xinjiang,China;Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Liaoning Agricultural Development Service Center,Shenyang 110003,Liaoning,China;Panjin Modern Agriculture Development Center,Panjin 124000,Liaoning,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2023年第7期2624-2633,共10页
Chinese Journal of Biotechnology
基金
国家重点研发计划(2018YFC1200602,2018YFC1200502)。
关键词
猪流行性腹泻
mRNA疫苗
受体结合区
体液免疫
细胞免疫
porcine epidemic diarrhea
mRNA vaccine
receptor-binding region
humoral immune response
cellular immune response
