基于UPLC-MS技术分析铁皮石斛炮制前后糖类成分差异

UPLC-MS metabonomics technology revealing difference of carbohydrate in Dendrobium officinale under different processing methods

目的研究铁皮石斛Dendrobium officinale鲜条及其炮制品中糖类化合物,探讨铁皮石斛炮制前后对糖类化合物积累的影响,为铁皮石斛炮制工艺提供基础研究。方法采用超高效液相色谱-串联高分辨质谱(UPLC-MS)非靶向技术对铁皮石斛鲜条及其炮制品代谢物质进行检测,使用主成分分析(principal component analysis,PCA)和正交偏最小二乘判别分析法(orthogonal partial least squares-discriminant analysis,OPLS-DA)鉴定代谢物中糖类化合物的差异代谢物。结果共鉴定76个糖类化合物,61个糖类化合物在西枫斗中上调积累。其中麦芽五糖和麦芽六糖炮制前后差异倍数达30倍,15个糖类化合物及其团聚体下调积累,炮制前后下调的差异倍数幅度较小。结论炮制明显提升铁皮石斛炮制品西枫斗中代谢物的积累,研究结果为西枫斗炮制的深入研究提供理论基础。

Objective To study the sugar compounds in raw Dendrobium officinale and its processed products,to explore the effect of D.officinale on the accumulation of sugar compounds before and after processing,and to provide preliminary applied basic research for the processing technology of D.officinale.Xifengdou.Methods High liquid chromatography-tandem mass spectrometry(UPLC-MS)non-targeted technology was used to detect the metabolites of D.officinale and its processed products.Principal component analysis(PCA)and orthogonal partial minimum Orthogonal partial least squares-discriminant analysis(OPLS-DA)was used to identify differential metabolites of carbohydrates in metabolites.Results A total of 76 carbohydrates were identified,and 61 carbohydrates were up-regulated and accumulated in Xifengdou.Among them,maltopentaose and maltohexaose had a 30-fold difference before and after processing,and 15 sugar compounds and their aggregates were down-regulated and accumulated,and the difference in the fold before and after processing was small.Conclusion Processing significantly increases the accumulation of metabolites in Xifengdou,a processed D.officinale product.The research results provide a theoretical basis for the in-depth study of Xifengdou concoction.