载丹皮酚纳米纤维膜的制备、表征及性能评价

Preparation,characterization and performance evaluation of nanofiber membranes loaded with paeonol

目的 以丹皮酚、聚己内酯(polycaprolactone)、明胶(gelatin)为原料,利用静电纺丝技术制备载药纤维膜,并进行结构表征及性能评价。方法 采用静电纺丝仪制备了聚己内酯/丹皮酚纤维膜、聚己内酯+明胶/丹皮酚纤维膜以及三层纤维膜(上下层为聚己内酯膜,中间层为聚己内酯+明胶/丹皮酚膜)。通过扫描电子显微镜、傅里叶变换红外光谱仪、X射线衍射仪、差示量热扫描仪对单层纤维膜的形貌与结构进行表征。通过CCK-8实验和溶血率测定法对载药纤维膜的安全性进行初步评价;并测定了聚己内酯+明胶/丹皮酚纤维膜的抗菌活性和抗氧化性;通过体外释放实验考察丹皮酚的释放规律。结果 丹皮酚成功负载于电纺纤维中,CCK-8实验测得聚己内酯+明胶/丹皮酚纤维膜(载药量≤15%)对L929细胞存活率并无明显影响,溶血率实验测得样品纤维膜的溶血率都低于1%。聚己内酯+明胶/丹皮酚纤维膜对大肠杆菌和金黄色葡萄球菌Staphylococcus aureus具有良好的抑菌效果,其中对金黄色葡萄球菌的抑菌圈直径为(19.93±0.10)mm。聚己内酯+明胶/丹皮酚载药纤维膜对羟自由基的清除力较强,当丹皮酚的质量浓度为1.1 mg/mL,载药纤维膜的清除率为78.72%。体外释放实验表明药物的释放先是经过一个快速释放阶段后进入缓慢释放过程。3层纤维膜的缓释效果最好,累积释放率为94.58%。结论 制备的丹皮酚纤维膜具有较好的生物相容性和抑菌活性及抗氧化活性,具有缓释性,其在生物医药领域的应用值得进一步研究。

Objective To prepare drug-loaded fiber membrane by using electrospin technology,with paeonol,polycaprolactone,gelatin as raw materials and performe their structural characterization and performance evaluation.Methods Polycaprolactone/paeonol fiber membrane,polycaprolactone + gelatin/paeonol fiber membrane and three layers of fiber film(which polycaprolactone membrane as the upper and lower layers and polycaprolactone + gelatin/paeonol membrane as the middle layer) were prepared.The morphology and structure of the single layer fiber membrane were characterized by scanning electron microscope,Fourier transform infrared spectrum,X-ray diffraction,and differential scanning calorimetry.The security of the loaded fiber membrane was assessed by CCK-8 and hemolysis test.The antibacterial activity and antioxidant properties of polycaprolactone + gelatin/paeonol were evaluated.The in vitro release rule of paeonol from drug loaded fiber membrane was investigated.Results Paeonol was successfully loaded in the electrospun fibers.CCK-8 test indicated that the polycaprolactone + gelatin/paeonol fiber membrane(drug loading capacity ≤ 15%)showed no influence to the cell viability,and hemolysis test showed hemolysis ratio of samples was under 1%.The excellent antibacterial effect of polycaprolactone + gelatin/paeonol fiber membrane against Escherichia coli and Staphylococcus aureus,which the diameter of the bacteriostatic ring for S.aureus was(19.93 ± 0.10) mm.Polycaprolactone + gelatin/paeonol had a strong clearance of hydroxyl radicals,when the concentration of paeonol was 1.1 mg/mL,the clearance rate of the membrane was 78.72%.In vitro release experiments show that the release of the drug first goes through a rapid release and then enters the slow release.The fiber membrane with the best sustained-release effect was three layers,which the cumulative release rate was 94.58%.Conclusion The prepared paeonol fiber membrane has good biocompatibility,bacteriactivity and oxidation resistance,and slow release,and its application in the field of biomedicine is worth further study.