基于高通量测序技术的细菌型火锅蘸料菌群结构研究

Study on the Structure of Bacterial Hot Pot Dipping Microflora Based on High throughput Sequencing Technology

采用高通量测序对4种不同处理方式火锅蘸料的细菌菌群结构,结合丰度聚类热图和α多样性进行分析。测序的4个样本共得到142398条有效数据,18770个OTUs。通过高通量基因测序技术,对4种不同处理方式火锅蘸料细菌菌群结构和丰度进行鉴定,分析火锅蘸料中可能影响火锅蘸料品质的菌株,为火锅蘸料的安全生产提供科学依据。在火锅蘸料生产中,通过鉴定火锅蘸料中微生物组成,可在生产中针对这些微生物的生存特性抑制有害微生物。研究目的是利用高通量测序方法对不同火锅蘸料的细菌菌群结构和组成进行分析比较,以期探索火锅蘸料中的有害微生物,实现对火锅蘸料中有害菌的防治,提升加工水平并为工艺及设备改进提供科学依据,从而实现提高火锅蘸料的品质。

High throughput sequencing was used to analyze the bacterial flora structure of hot pot dipping materials with 4 different treatment methods,combined with the abundance clustering heat map andαdiversity analysis.A total of 142398 valid data and 18770 OTUs were obtained from 4 samples in this sequencing.Through high-throughput gene sequencing technology,the bacterial flora structure and abundance of four different hot pot dips were identified,and the strains in hot pot dips that may affect the quality of hot pot dips were analyzed,providing scientific basis for the safe production of hot pot dips.In the future hot pot dip production,the identification of microbial composition in the hot pot dip could inhibit harmful microorganisms according to the survival characteristics of these microorganisms.The purpose of this study was to analyze and compare the bacterial flora structure and composition of different hot pot dips by high-throughput sequencing method,so as to explore the harmful microorganisms in hot pot dips,to achieve the prevention and control of harmful bacteria in hot pot dips,to improve the processing level and to provide scientific basis for the improvement of technology and equipment,so as to improve the quality of hot pot dips.