五布红柚类胡萝卜素物质的提取鉴定及焦甜香效果研究

Extraction and Identification of Carotenoids from Citrus grandis Osbeck cv.Wubuhongyou and Study on the Effect of Burning Sweet Flavor

建立高效液相色谱法测定检测五布红柚中类胡萝卜素物质含量的方法。样品用丙酮和甲醇(丙酮与甲醇7︰3,V/V;0.1%BHT W/V)提取,完全溶解在甲醇/乙腈/正己烷(7︰2︰1,V/V)混合溶液中。分离柱为Poroshell 120 EC-C_(18)柱(4.6 mm×150 mm,4μm),流动相为乙腈、甲醇和正己烷溶液,梯度洗脱,流速1.0 mL/min,柱温25℃,进样量50μL,检测波长450 nm。检测方法显示:5种类胡萝卜素物质的检测线性范围为0.02~500 mg/L;检出限(rSN=3)在0.003~0.03 mg/kg,加标回收率(N=6)在86.39%~105.96%,该方法灵敏、准确、快速、准确、高效。检测结果表明,不同产地得到的五布红柚提取物中番茄红素、β-胡萝卜素、玉米黄素、α-胡萝卜素、花药黄素5种五布红柚提取物类胡萝卜素物质的总含量以重庆巴县产五布红柚提取物最高。烟丝中加香试验评吸结果表明,焦甜香增香效果也较为明显。

A high performance liquid chromatography method was established to determine and detect the carotenoid content of Citrus grandis Osbeck cv.Wubuhongyou.The sample was extracted with acetone and methanol(acetone and methanol 7︰3,V/V;0.1%BHT W/V)and completely dissolved in the mixed solution of methanol/acetonitrile/n-hexane(7︰2︰1,V/V).The separation column was a Poroshell 120 EC-C_(18) column(4.6 mm×150 mm,4μm),and the mobile phase is acetonitrile,methanol and n-hexane solution,with gradient elution,the flow rate of 1.0 mL/min,the column temperature of 25℃,the injection volume of 50μL,and the detection wavelength of 450 nm.The detection method showed that the detection linear range of five carotenoids was 0.02-500 mg/L.The detection limit(rSN=3)was between 0.003 mg/kg and 0.03 mg/kg,and the recovery rate(N=6)was between 86.39%and 105.96%.This method was sensitive,accurate,rapid,accurate and efficient.The results showed that the total content of carotenoids in five kinds of extracts from Citrus grandis Osbeck cv.Wubuhongyou,including lycopene,β-carotene,zeaxanthin,α-carotene and anther yellow,was the highest in Citrus grandis Osbeck cv.Wubuhongyou from Baxian County,Chongqing.The evaluation results of the aroma adding test in cut tobacco showed that the aroma enhancement effect of burnt sweet aroma was also relatively obvious.