氢吗啡酮对大鼠的脑保护作用

Cerebral protective effect of hydromorphone in rats

目的探讨氢吗啡酮对大鼠脑缺血再灌注损伤的影响。方法45只SD雄性大鼠随机分成3组:假手术组(Sham组)、脑缺血再灌注组(I/R组)和氢吗啡酮组(HM组)。采用Zea-Longa改良线拴法构建动物模型,再灌注24 h后,Zea-Longa评分法评价神经功能;TTC染色检测脑梗死体积;苏木精-伊红(HE)和Nissl染色观察海马神经元病理变化,Tunel染色观察细胞凋亡情况,Western blot、qPCR检测凋亡相关因子B淋巴细胞瘤(Bcl)-2、Bcl-2相关X蛋白(Bax)和半胱氨酸蛋白酶(Caspase)-3蛋白和mRNA表达量。结果与I/R组相比,HM组神经功能评分下降和脑梗死面积减小(P<0.05),Tunel阳性细胞数量减少(P<0.05),Bax和Caspase-3蛋白mRNA表达量减少,而Bcl-2表达量显著增加(P<0.05)。结论氢吗啡酮具有神经保护作用,可减轻大鼠脑缺血再灌注损伤。

Objective To investigate the effect of hydromorphone on cerebral ischemia-reperfusion injury in rats.Methods Forty-five SD male rats were randomly divided into three groups:sham-operated group(Sham group),cerebral ischemia-reperfusion group(I/R group)and hydromorphone group(HM group).The animal models were constructed using the Zea-Longa modified line tethering method,and neurological function was evaluated by the Zea-Longa score after 24 h of reperfusion.TTC staining was used to detect the volume of cerebral infarction,hematoxylin-eosin(HE)and Nissl staining were used to observe the pathological changes of hippocampal neurons,and Tunel staining was used to observe apoptosis,Western blot,qPCR were used to detect apoptosis Bcl-2,Bcl-2-associated X protein(Bax)and cysteine protease(Caspase)-3 protein and mRNA expression.Results Compared with the I/R group,the HM group showed lower neurological function scores and cerebral infarct area(P<0.05),smaller number of Tunel-positive cells(P<0.05),less mRNA expression of Bax and Caspase-3 proteins and significantly higher expression of Bcl-2(P<0.05).Conclusions Hydromorphone has neuroprotective effects and can reduce cerebral ischemia-reperfusion injury in rats.