2021年上海市嘉定区生禽肉中沙门菌污染状况及病原学特征分析

Analysis of Salmonella contamination and pathogenic characteristics in raw poultry meats in Jiading District,Shanghai in 2021

【目的】了解上海市嘉定区2021年生禽肉中沙门菌污染状况、血清型分布及耐药性。【方法】采集鸡、鸭、鹅和鸽4类生禽肉样本进行沙门菌分离鉴定,并对分离的沙门菌进行血清型鉴定和16种常见抗生素耐药性检测。【结果】80份生禽肉样品中分离到沙门菌60株。鸽肉、鹅肉、鸭肉和鸡肉中沙门菌的检出率分别为86.67%、81.82%、72.73%、59.38%。冷冻、冷藏和室温存储样品中,冷藏存储的样品检出率最低,为65.63%。血清型以鼠伤寒沙门菌、肠炎沙门菌和科瓦利斯沙门菌为主。四环素、萘啶酸、氨苄西林、氯霉素4类抗生素耐药率较高,分别为60.00%、58.33%、50.00%、48.33%;头孢噻肟耐药率低,对亚胺培南完全敏感。不同类别生禽肉中检出的沙门菌及不同型别的沙门菌耐药差异明显。11.67%的沙门菌为不耐药,多重耐药菌株占比为46.67%,耐药数最多达13种。氨苄西林-四环素或氯霉素-头孢唑林耐药模式提示菌株为多重耐药菌株。【结论】上海市嘉定区生禽肉样本沙门菌的污染状况严重,耐药性升高,应加强监测并进行控制。

[Objective]To investigate the contamination status,serotype distribution and drug resistance of Salmonella in retail poultry in Jiading District,Shanghai.[Methods]Four types of poultry(chicken,duck,goose and pigeon)have been sampled from commercial markets.Salmonella contamination has been isolated and identified using serotype analysis.Furthermore,resistance of isolated Salmonella strains towards 16 commonly used antibiotics has been determined.[Results]A total of 60 Salmonella strains were isolated from 80 poultry samples.The detection rates of Salmonella in pigeon,goose,duck and chicken were 86.67%,81.82%,72.73%and 59.38%,respectively.Contamination status has been categorized by storage conditions.Lowest detection rate(65.63%)has been noted in poultry samples under refrigeration storage.The majority serotypes of Salmonella have been revealed as Salmonella typhimurium,Salmonella enteritidis and Salmonella corvallis.Relatively higher drug resistance was discovered with tetracycline,nalidixic acid,ampicillin and chloramphenicol,with drug resistance rate of 60.00%,58.33%,50.00%and 48.33%,respectively.Low drug resistance was revealed with cefotaxime.In addition,these Salmonella strains were completely sensitive to imipenem.Significant difference in drug resistance was identified across the types of poultry or Salmonella serotypes.The 11.67%of Salmonella strains were non-resistant to any tested drugs.Multi-drug resistant strains accounted for 46.67%of isolated strains,which were resistant to 13 different antibiotics.Ampicillin-tetracycline or chloramphenirol-cefazolin drug resistance pattern suggested that the Salmonella strain was multi-drug resistant.[Conclusion]Salmonella contamination remains high in retail poultry in Jiading District,Shanghai.Drug resistance to antibiotics is increasing.Therefore,monitoring and control of Salmonella should be strengthened.