摘要
小菜蛾(Plutella xylostella)肠道中有丰富的微生物,易受食物及饲养环境影响。为了解抗生素在饲养过程中对小菜蛾肠道菌群的影响,用氨苄青霉素、硫酸链霉素和利福平3种抗生素浸泡甘蓝叶后饲喂小菜蛾,采用高通量测序技术研究其肠道微生物群落结构的变化。结果表明,小菜蛾肠道细菌主要为厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)和拟杆菌门(Bacteroidota),肠球菌属(Enterococcus)和肠杆菌属(Enterobacter)为优势属;真菌主要为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)和毛霉门(Mucoromycota),优势属为青霉属(Penicillium)。小菜蛾取食不同剂量抗生素浸泡的甘蓝叶后,肠道中子囊菌门相对丰度均上升,厚壁菌门相对丰度均下降;担子菌门在10 mg/L氨苄青霉素处理后相对丰度显著降低,其次是100 mg/L硫酸链霉素处理组,其余处理组均上升;毛霉门在100 mg/L硫酸链霉素处理后相对丰度显著提升,其次是5 mg/L氨苄青霉素处理组,其余处理组均下降;变形菌门在100 mg/L硫酸链霉素处理后相对丰度降低,其余处理组均上升;拟杆菌门相对丰度在100 mg/L硫酸链霉素处理下上升最多。属水平上,青霉属相对丰度均提升,肠球菌属相对丰度均降低,肠杆菌属相对丰度除100 mg/L硫酸链霉素处理组外均增加。取食3种抗生素浸泡的甘蓝叶后小菜蛾肠道真菌群落丰富度、均匀度和多样性均降低,其中10 mg/L氨苄青霉素处理组降幅最大;细菌群落均匀度和多样性增加,其中100 mg/L硫酸链霉素处理组增幅最大,群落丰富度经氨苄青霉素和硫酸链霉素处理后增加,而经利福平处理后降低。
The gut of Plutella xylostella(Lepidoptera:Plutellidae)is abundant with microorganisms that are susceptible to the effects of food and feeding environment.To understand the effects of antibiotics on the gut flora of P.xylostella during feeding,the changes of the gut microbial community of P.xylostella after cabbage leaves were treated with ampicillin,streptomycin sulfate and rifampicin and used to feed P.xylostella larvae,were investigated using high-throughput sequencing technology in this paper.The results showed that the gut bacteria of P.xylostella were mainly Firmicutes,Proteobacteria and Bacteroidota,and the dominant genera were Enterococcus and Enterobacter.The gut fungi of P.xylostella were mainly Ascomycota,Basidiomycota and Mucoromycota,and the dominant genus was Penicillium.After treatment with each antibiotic,the relative abundance of Ascomycota all increased,and that of Firmicutes all decreased.The relative abundance of Basidiomycota significantly decreased after 10 mg/L ampicillin treatment,followed by 100 mg/L streptomycin sulfate treatment,while the relative abundance all increased in other groups.The relative abundance of Mucoromycota significantly increased after 100mg/L streptomycin sulfate treatment,followed by 5 mg/L ampicillin sulfate,while the relative abundance all decreased in other groups.The relative abundance of Proteobacteria decreased after 100 mg/L streptomycin sulfate treatment and the relative abundance all increased in other groups.The relative abundance of Bacteroidota increased the most under 100 mg/L streptomycin sulfate treatment.At genus level,the relative abundance of Penicillium all increased,the relative abundance of Enterococcus all decreased,and the relative abundance of Enterobacter increased in all groups except for the 100 mg/L streptomycin sulfate treatment.The abundance,evenness and diversity of the fungal community in the gut of P.xylostella were all decreased for all three antibiotics treatments,with the highest decrease in 10 mg/L ampicillin treatment.The evenness and diversity of bacterial community all increased for all three antibiotics treatments,with the highest increase in the treatment of 100 mg/L streptomycin sulfate.The bacterial abundance increased for both treatments of ampicillin and streptomycin sulfate,while decreased for rifampicin treatment.
作者
李文红
郭涛
向立刚
余知和
李添群
LI Wenhong;GUO Tao;XIANG Ligang;YU Zhihe;LI Tianqun(Institute of Plant Protection,Guizhou Academy of Agricultural Sciences,Guiyang 550081,China;College of Life Sciences,Yangtze University,Jingzhou 434025,China;College of Agriculture,Yangtze University,Jingzhou 434025,China;Bureau of Agriculture and Rural of Xiuwen,Xiuwen 550299,China)
出处
《河南农业科学》
北大核心
2023年第7期98-108,共11页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金项目(31860509)
黔农科院国基后补助项目[(2021)36号]。
关键词
小菜蛾
肠道微生物菌群
氨苄青霉素
硫酸链霉素
利福平
高通量测序
Plutella xylostella
Gut microbial flora
Ampicillin
Streptomycin sulfate
Rifampicin
High-throughput sequencing