黄花菜维生素E含量遗传分析及QTL定位

Genetic analysis and QTL localization of vitamin E content in Hemerocallis citrina Baroni

维生素E是黄花菜中重要的营养成分。本研究以黄花菜地方品种‘东庄黄花’和‘冲里花’杂交获得的F1群体为研究对象,对杂交后代连续2年的维生素E含量进行检测分析,结果显示黄花菜F1群体维生素E含量的变异系数为50.44%~54.23%,变异系数较大,整体呈现正态分布趋势,并出现了含量超过双亲的超亲后代个体;遗传分析显示黄花菜维生素E含量符合2对主基因-加性-显性模型(2MG-AD),第1对基因加性效应更为显著,主基因遗传效率高达95%以上;对黄花菜维生素E含量进行QTL定位,共检测到2个与黄花菜维生素E相关的QTL,分布于8号和10号连锁群上,LOD峰值的范围为2.96~3.60,单个QTL贡献率介于10.80%~13.00%。本研究结果为黄花菜高维生素E含量的新品种选育和品种改良提供了数据基础。

Vitamin E is an important nutrient in Hemerocallis citrina Baroni.In this study,the F1 population obtained by crossing the local variety of Hemerocallis citrina Baroni‘Dongzhuang Huanghua’and‘Chonglihua’was used as the research object,and the vitamin E content of the cross progeny was examined and analyzed for 2 consecutive years.The results showed that the vitamin E content in the F1 pulation of Hemerocallis citrina Baroni displayed an normal distribution with a large coefficient of variation that ranged from 50.44%to 54.23%.There were super-parental progeny whose content exceeded that of both parents.Genetic analysis showed that the vitamin E content of Hemerocallis citrina conformed to the additive-dominance model controlled by two major genes(2MG-AD).The genetic efficiency of the main genes are over 95%.QTL analysis has identified two locus related to vitamin E in Hemerocallis citrina Baroni.The two locus distributed on the linkage group 8 and 10,and the range of LOD peak was 2.96-3.60. The contribution rate of individual QTL ranged from 10.80% to 13.00%. The results of this study provide a data base for the selection of new varieties and variety improvement of Hemerocallis citrina Baroni with high vitamin E content.