纳米孔测序在肿瘤并发感染中的应用

Application of Nanopore Sequencing in Tumor with Infection

目的评估纳米孔测序在肿瘤患者并发感染性疾病中的临床应用价值,为肿瘤患者感染性疾病的快速、灵敏诊断提供新的检测方法。方法收集2022年7月至8月云南省肿瘤医院36例疑似感染的肿瘤患者样本46份,利用纳米孔测序鉴定样本中的病原微生物。结果纳米孔测序细菌检出率72.22%,真菌检出率31.48%,病毒检出率42.59%。细菌和真菌检出率均较微生物培养法(23.91%和10.87%)明显升高(χ^(2)=23.19,P<0.0001;χ^(2)=13.14,P=0.0003)。纳米孔测序结果与微生物培养结果比较,检出的细菌和真菌种属明显增多。结论纳米孔测序鉴定病原微生物具有检出率高,检测病原菌种属广,诊断快速的优势,在肿瘤并发感染性疾病的诊断中具有潜在的临床应用价值。

Objective To evaluate the clinical application value of nanopore sequencing in tumor patients with concurrent infectious diseases and to provide a new detection method for rapid and sensitive diagnosis of infectious diseases in tumor patients.Methods Samples of inpatients with suspected infections from July to August 2022 at Yunnan Cancer Hospital were collected,and pathogenic microorganisms in the samples were identified using nanopore sequencing.Results Forty-six samples from 36 patients with suspected infection were collected.Nanopore sequencing detected 72.22%of bacteria,31.48%of fungi,and 42.59%of viruses.Both bacterial and fungal detection rates were significantly higher(χ^(2)=23.19,P<0.0001;χ^(2)=13.14,P=0.0003)than the results by microbiological culture method(23.91%and 10.87%).The nanopore sequencing results showed significantly more bacterial and fungal species detected compared to microbial culture results.Conclusions Nanopore sequencing for the identification of pathogenic microorganisms has the advantages of high detection rate,wide detection of pathogenic genera,and rapid diagnosis.It has potential clinical application in the diagnosis of tumor-associated infectious diseases.