不同月龄A53T小鼠背根神经节中α-Syn表达变化

EXPRESSION OFα-SYNUCLEIN IN THE DORSAL ROOT GANGLION OF A53T MICE WITH DIFFERENT AGES IN MONTHS

目的探讨不同月龄A53T转基因(A53T)小鼠脊髓背根神经节(DRG)中α-突触核蛋白(α-Syn)表达水平的变化。方法6月龄和12月龄野生型(WT)小鼠和A53T小鼠经乌拉坦腹腔麻醉后处死,在体视显微镜下取出胸段、腰段和荐段的DRG组织,采用蛋白免疫印迹法检测DRG中α-Syn的表达水平。结果与相同月龄WT小鼠相比,6月龄及12月龄A53T小鼠DRG中α-Syn表达量显著增加(F=59.164、55.681,P<0.01);与6月龄A53T小鼠相比,12月龄A53T小鼠DRG中α-Syn的表达量显著增加(F=13.802,P<0.05)。结论A53T小鼠DRG中α-Syn表达量高于同月龄WT小鼠,且其表达量随年龄增长而增加。

Objective To investigate the expression level ofα-synuclein(α-Syn)in the dorsal root ganglion(DRG)of A53T transgenic mice with different ages in months.Methods Wild-type(WT)mice and A53T mice,aged 6 or 12 months,were sacrificed after intraperitoneal anesthesia with urethane.DRG tissue samples of the thoracic,lumbar,and sacral segments were collected under a stereomicroscope,and Western blotting was used to measure the expression level ofα-Syn.Results Compared with the WT mice with the same age in months,the A53T mice aged 6 or 12 months had a significant increase in the expression level ofα-Syn in DRG(F=59.164,55.681;P<0.01).Compared with the A53T mice aged 6 months,the A53T mice aged 12 months had a significant increase in the expression level ofα-Syn in DRG(F=13.802,P<0.05).Conclusion A53T mice have a higher expression level ofα-Syn in DRG than WT mice with the same age in months,and the expression level ofα-Syn increases with age.