大鼠牙髓组织AIM2炎症体表达及其与炎症反应关系

EXPRESSION OF ABSENT IN MELANOMA 2 INFLAMMATORY BODY IN RAT DENTAL PULP AND ITS ASSOCIATION WITH INFLAMMATORY RESPONSE

目的探讨黑色素瘤缺乏因子2(AIM2)炎症体在大鼠牙髓组织中的分布及其在牙髓炎症发生、发展中的表达变化。方法通过开髓法建立大鼠牙髓炎症模型,采用免疫荧光染色检测AIM2炎症体在牙髓组织中的表达分布;利用实时定量PCR(RT-PCR)检测大鼠牙髓组织中AIM2炎症体基因表达量的变化。结果苏木精-伊红(HE)染色显示单纯的开髓可诱发大鼠牙髓炎症反应,开髓3 d后出现炎性反应并在7 d后出现牙髓坏死。免疫荧光染色显示,大鼠牙髓组织中有AIM2炎症体表达,开髓5 d后牙髓细胞层出现明显阳性表达。RT-PCR结果显示,开髓术后3 d AIM2基因表达显著增加(F=1178.910,P<0.01),下游caspase-1基因表达上调(F=81.224,P<0.05),IL-1β在开髓5 d后显著上调(F=164.414,P<0.05)。结论AIM2炎症体存在于大鼠牙髓组织中,并且其表达量与牙髓炎症反应呈正相关。

Objective To investigate the distribution of absent in melanoma 2(AIM2)inflammatory body in rat dental pulp tissue and the change in its expression in the development and progression of pulpitis.Methods Opening of pulp chamber was performed to establish a rat model of dental pulp inflammation.Immunofluorescent staining was used to measure the expression and distribution of AIM2 inflammatory body in dental pulp tissue,and RT-PCR was used to measure the change in the mRNA expression level of AIM2 inflammatory body in rat dental pulp tissue.Results HE staining showed that opening of pulp chamber alone could induce inflammatory response of rat dental pulp;inflammatory response was observed on day 3 after pulp opening and dental pulp necrosis was observed on day 7 after pulp opening.Immunofluorescent staining showed the expression of AIM2 inflammatory body in rat dental pulp tissue,with marked positive expression in pulp cell layer on day 5 after pulp opening.RT-PCR showed that on day 3 after pulp opening,there were significant increases in the expression levels of the AIM2 gene(F=1178.910,P<0.01)and downstream caspase-1(F=81.224,P<0.05),and there was a significant increase in IL-1βon day 5 after pulp ope-ning(F=164.414,P<0.05).Conclusion AIM2 inflammatory body exists in rat dental pulp tissue,and the changing trend of its expression is positively associated with dental pulp inflammatory response.