凝结芽孢杆菌(Bacillus coagulans)CGMCC 9951提升运动耐力作用的研究

Effects of Bacillus coagulans CGMCC 9951 on improving sports endurance

为探究凝结芽孢杆菌CGMCC 9951对小鼠运动耐力的影响,将50只小鼠随机分为5组:安静对照(SC)组、运动模型对照(EC)组、凝结芽孢杆菌低剂量(LC)组、中剂量(MC)组、高剂量(HC)组。通过4周游泳训练建立小鼠运动模型,期间每天补充凝结芽孢杆菌CGMCC 9951,测量小鼠体重和主要脏器重量、游泳力竭时间、血清生化指标(血糖、乳酸、血氨、血尿素氮、乳酸脱氢酶、肌酸激酶)、肝糖原和肌糖原、炎症因子(白细胞介素-1β、白细胞介素-10、肿瘤坏死因子-α)含量。与EC组相比,补充凝结芽孢杆菌CGMCC 9951能够显著延长HC组小鼠游泳力竭时间,提升运动后血糖水平,提高肝糖原和肌糖原的储备量;血清中乳酸、血氨含量显著降低,乳酸脱氢酶和肌酸激酶活性减弱;凝结芽孢杆菌CGMCC 9951能够显著降低促炎因子(白细胞介素-1β、肿瘤坏死因子-α)和提高抗炎因子(白细胞介素-10)的含量。凝结芽孢杆菌CGMCC 9951可提高小鼠的运动耐力能力,显著降低血清炎症水平,缓解机体疲劳,在益生菌制剂的开发中具有良好的应用潜力。

In this study,a mouse exercise model was constructed by four weeks of swimming training to explore the effect of Bacillus coagulans CGMCC 9951 on exercise endurance in mice.50 mice were randomly divided into five groups:the quiet control(SC)group,the exercise control(EC)group and three groups treated with Bacillus coagulans CGMCC 9951 of low dose(LC),medium dose(MC)and high dose(HC).The mouse exercise model was established by swimming training for four weeks,and B.coagulans CGMCC 9951 was supplemented daily.The basic parameters,swimming exhaustion time and serum biochemical indexes(blood glucose,lactic acid,blood ammonia,blood urea nitrogen,lactate dehydrogenase,creatine kinase)liver glycogen and muscle glycogen were measured,as well as inflammatory factors,i.e.interleukin-1β(IL-1β),interleukin-10(IL-10),tumor necrosis factor-α(TNF-α).Results showed that compared with EC group,the supplementation of B.coagulans CGMCC 9951 significantly prolonged the exhaustive swimming time of mice in HC group,increased the blood glucose level,liver glycogen and muscle glycogen.It was observed that the level of lactic acid and blood ammonia decreased significantly in serum.Additionally,B.coagulans CGMCC 9951 significantly reduced the content of pro-inflammatory factors(IL-1β,tumor TNF-α)while that of anti-inflammatory factors(IL-10)increased.B.coagulans CGMCC 9951 could enhance exercise tolerance and relieve fatigue in mice,which has potential application in the development of probiotics.