摘要
An efficient enzymatic assembly strategy was developed for the concise synthesis of structurally well-defined sialylated chitooligosaccharides.Two enzyme modules for theβ-1,3-N-acetyl-glucosaminylation andβ-1,4-galactosylation were applied for the grafting N-acetyl lactosamine(LacNAc)unit(s)onto the chitooligosaccharides.The LacNAc grafted chitooligosaccharides were further modified withα-2,3-orα-2,6-sialylation by two enzymatic sialylation modules to generate a total of 20 sialylated chitooligosaccharides.The inhibition study of influenza virus-induced cytopathy with synthetic sialylated chitooligosaccharides indicated that the sialic acid linkage and chain length both contribute to the binding preference and inhibition potency.
基金
supported by the National Key Research and Development Program of China(2021YFC210500 and 2022YFC2104900)
the National Natural Science Foundation of China(21961142016,22277111 and 22107094)
Department of Science and Technology of Shandong Province(2020CXGC010601,2021ZDSYS22 and ZR2021QB061)。
