非小细胞肺癌中OGDHL、富含AU元件的RNA结合蛋白1的表达及其与预后的相关性

Expressions of OGDHL and AU element-rich RNA-binding protein 1 in non-small cell lung cancer and their correlation with prognosis

目的:探讨OGDHL、富含AU元件的RNA结合蛋白1(AUF1)在非小细胞肺癌(NSCLC)患者中的表达,及其与患者预后的相关性。方法:回顾性分析2018年2月至2019年2月在广安市人民医院确诊并进行手术治疗的96例NSCLC患者的临床资料。取手术切除的NSCLC肿瘤组织及距肿瘤边缘4 cm处的癌旁组织,采用免疫组织化学法检测所有标本组织中OGDHL、AUF1蛋白表达情况。分析OGDHL、AUF1蛋白与NSCLC临床病理特征关系,采用Kaplan-Meier法比较不同OGDHL、AUF1蛋白表达患者的3年总生存(OS)率;采用多因素Cox比例风险模型分析NSCLC患者预后的影响因素。结果:NSCLC患者肿瘤组织OGDHL蛋白高表达率低于癌旁组织[32.3%(31/96)比62.5% (60/96), χ^(2)=45.21, P<0.001],AUF1蛋白高表达率高于癌旁组织[68.8%(66/96)比34.4%(33/96), χ^(2)=42.36, P<0.001]。TNM分期Ⅰ~Ⅱ期患者OGDHL蛋白高表达率高于Ⅲ~Ⅳ期[39.1%(25/64)比18.8%(6/32)],有淋巴结转移患者OGDHL蛋白高表达率低于无淋巴结转移[10.3%(3/29)比41.8%(28/67)](均 P<0.05);Ⅰ~Ⅱ期患者AUF1蛋白高表达率低于Ⅲ~Ⅳ期患者[59.4%(38/64)比87.5%(28/32)],有淋巴结转移患者AUF1蛋白高表达率高于无淋巴结转移患者[86.2%(25/29)比61.2% (41/67)](均 P<0.05)。所有NSCLC患者3年OS率为62.50%。其中OGDHL蛋白高表达组患者术后3年OS率高于低表达组(80.66%比53.33%, P<0.05),AUF1蛋白高表达组患者术后3年OS率低于低表达组(50.00%比76.52%, P<0.05)。不同TNM分期、是否淋巴结转移的NSCLC患者3年OS率差异均有统计学意义(均 P<0.05),且TNM分期Ⅲ~Ⅳ期、有淋巴结转移患者3年OS率均分别低于Ⅰ~Ⅱ期、无淋巴结转移的患者(均 P<0.05)。OGDHL低表达( HR=3.78,95% CI 1.72~8.31)、AUF1高表达( HR=3.67,95% CI 1.73~7.80)均是影响NSCLC患者预后的独立危险因素(均 P<0.001)。 结论:NSCLC组织中OGDHL蛋白表达降低,AUF1蛋白表达升高,且二者表达水平与NSCLC患者预后相关。

ObjectiveTo explore the expression of OGDHL and AU element-rich RNA-binding protein 1(AUF1)in patients with non-small cell lung cancer(NSCLC)and their correlation with the prognosis of patients.MethodsThe clinical data of 96 patients with NSCLC who were diagnosed and underwent surgical treatment in Guang'an People's Hospital from February 2018 to February 2019 were retrospectively analyzed.The surgically resected NSCLC tissues and the paracancerous tissues(4 cm from the tumor edge)were taken,and the immunohistochemical staining was used to detect the protein expressions of OGDHL and AUF1 in all specimens.The relationship between OGDHL and AUF1 proteins and clinicopathological characteristics of NSCLC was analyzed.The 3-year overall survival(OS)rate of patients with different expressions of OGDHL and AUF1 proteins were compared by using Kaplan-Meier method.Multivariate Cox proportional hazards model was used to explore the influencing factors for the prognosis of NSCLC patients.ResultsThe high expression rate of OGDHL protein in the cancer tissues of NSCLC patients was lower than that in the paracancerous tissues[32.3%(31/96)vs.62.5%(60/96),χ^(2)=45.21,P<0.001],and the high expression rate of AUF1 protein was higher than that in the paracancerous tissues[68.8%(66/96)vs.34.4%(33/96),χ^(2)=42.36,P<0.001].The high expression rate of OGDHL protein in patients with TNM stageⅠ-Ⅱwas higher than that in patients with stageⅢ-Ⅳ[39.1%(25/64)vs.18.8%(6/32)],and the high expression rate of OGDHL protein in patients with lymph node metastasis was lower than that in patients without lymph node metastasis[10.3%(3/29)vs.41.8%(28/67)](both P<0.05).The high expression rate of AUF1 protein in patients with stageⅠ-Ⅱwas lower than that in patients with stageⅢ-Ⅳ[59.4%(38/64)vs.87.5%(28/32)],and the high expression rate of AUF1 protein in patients with lymph node metastasis was higher than that in patients without lymph node metastasis[86.2%(25/29)vs.61.2%(41/67)](both P<0.05).The 3-year OS rate of all NSCLC patients was 62.50%.The 3-year OS rate of patients with high expression of OGDHL protein after surgery was higher than that of patients with low expression of OGDHL protein(80.66%vs.53.33%,P<0.05),and the 3-year OS rate of patients with high expression group of AUF1 protein after surgery was lower than that of patients with low expression of AUF1 protein(50.00%vs.76.52%,P<0.05).The difference in 3-year OS rate of patients with different TNM stage and lymph node metastasis was statistically significant(both P<0.05),and the 3-year OS rate of patients with TNM stageⅢ-Ⅳand lymph node metastasis was lower than that of patients with stageⅠ-Ⅱand no lymph node metastasis(both P<0.05).Low expression of OGDHL(HR=3.78,95%CI 1.72-8.31)and high expression of AUF1(HR=3.67,95%CI 1.73-7.80)were both independent risk factors for the prognosis of NSCLC patients(both P<0.001).ConclusionsThe expression of OGDHL protein is decreased and the expression of AUF1 protein is increased in NSCLC tissues.The expression levels of OGDHL and AUF1 proteins are related to the prognosis of NSCLC patients.