猪伪狂犬病活疫苗(Bartha-K61株,传代细胞源)生产用毒种不同代次生物学特性的研究

Study on the Biological Characteristics of Different Generations of Porcine Pseudorabies Live Vaccine (Bartha K61 Strain,Cell Line Origin) in Swine Production

将猪伪狂犬病活疫苗(Bartha-K61株,传代细胞源)基础毒种F7传至F13,并将PRV F8~PRVF13六个代次毒种接种仔猪进行安全性检验,PRVF8、PRVF10、PRVF13三个代次毒种接种仔猪进行免疫原性检验,用以研究猪伪狂犬病活疫苗(Bartha-K61株,传代细胞源)生产用毒种不同代次生物学特性。安全性检验结果显示,各代次毒种经颈部肌肉接种仔猪,观察期内仔猪精神、食欲、体温均正常;免疫原性检验结果显示,免疫组仔猪攻毒后精神、食欲、体温均正常,对照组仔猪攻毒后体温升高,全部发病并死亡。以上结果表明,猪伪狂犬病活疫苗(Bartha-K61株,传代细胞源)不同代次的生产毒种对仔猪均安全且保持良好的免疫原性。

To study the biological characteristics of different generations of porcine pseudorabies live vaccine(Bartha-K61 strain,cell line origin)production virus seeds,the porcine pseudorabies live vaccine(Bartha-K61 strain,cell line origin)basic virus seed F7 was continuously passaged to F13,and six generations of PRV F8—PRV F13 were inoculated with piglets for safety test,and three generations of PRV F8、PRV F10 and PRV F13 were inoculated with piglets for immunogenicity test.The results of the safety test showed that the spirit,appetite,and body temperature of piglets were normal during the observation period after each generation of production virus seeds were inoculated on the neck muscles;the results of the immunogenicity test showed that the spirit,appetite,and body temperature of piglets in the immune group were normal after post-infection with PRV GD1 strain;while the body temperature of piglets in the control group was increased after post-infection with PRV GD1 strain,and all of them became ill and died.These results indicated that the different generations of porcine pseudorabies live vaccine(Bartha-K61 strain,cell line origin)production virus seeds were safe and maintained good immunogenicity to piglets.