巩固递进式动物细胞原代培养实验教学的构建与实践

Construction and practice of consolidated progressive experimental teaching of animal-cell primary culture

为解决传统实验教学中学生实验成功率低、能力素质锻炼不足的问题,对面向本科生的动物细胞原代培养实验教学进行改革,构建一种巩固递进式实验教学模式并付诸实践。该实验教学模式对重要且应用广泛的无菌技术、酶消法细胞原代培养技术进行多次巩固练习,并随实验项目的依次开展,逐步引入植块法细胞原代培养、免疫细胞化学鉴定对细胞原代培养实验进行递进深化,还适时安排学生进行先易后难的自主实验方案设计,实验的扩展性、深入性、综合性和设计性等均相应递增。学生的实验结果和教学效果问卷调查结果显示,该巩固递进式实验教学模式提高了学生的实验成功率,强化了学生实践、合作、分析和解决问题的能力,提高了实验教学质量。

The experiment of animal-cell primary culture for undergraduates was reformed in order to solve the problems of students’low success rate and insufficient training of abilities in traditional experimental teaching.Accordingly,a consolidated progressive experimental teaching mode was constructed and practiced.Aseptic technique and primary culture by enzymatic disaggregation,these important and widely applicated technologies,were practiced a lot.Primary culture by explantation and immunocytochemistry was phased to deepen the experiment teaching.Beyond that,students carried out the autonomous protocol designs,which were increasingly difficult.Extensibility,depth,comprehensiveness and design,therefore,were increased progressively with experiments.Students’experiment results and survey results about the teaching effect showed that this consolidated progressive experimental teaching mode effectively enhanced their success rate.Their abilities,including practice,cooperation,analysis/solution of problems,exploration and innovation,were strengthened.It effectively solved the above issues in traditional experimental teaching and correspondingly improved the quality of experimental teaching.