血清HMGA1、CXCL16水平与宫颈癌患者HPV感染分型的相关性分析

Correlation analysis of serum HMGA 1 and CXCL16 levels and classification of HPV infection in patients with cervical cancer

目的探究血清高迁移率族蛋白A1(HMGA1)、CXC亚家族趋化因子16(CXCL16)水平与宫颈癌患者人乳头瘤病毒(HPV)感染分型的相关性。方法回顾性选取2020年7月至2021年7月来广西医科大学附属第二医院妇科就诊的宫颈癌患者91例作为研究对象,检测所有患者的HPV感染分型情况。根据检查结果,45例患者(HPV感染基因型为HPV16、18、31、33、35、39、45、51、52、56、58、59、66、68等高危HPV分型)为高危组,46例患者(HPV感染分型为HPV6、11、42、43、44等)作为低危组,另选同期来广西医科大学附属第二医院进行体检的健康志愿者50名作为对照组。采用微粒子酶免疫分析法检测3组血清HMGA1水平进行,酶联免疫吸附试验检测3组血清CXCL16水平;采用Logistic回归分析分析血清HMGA1、CXCL16水平与宫颈癌患者HPV感染分型(高危型和低危组)的相关程度。结果高危组患者血清HMGA1、CXCL16水平分别为(156.23±16.14)ng/mL、(10.45±3.12)pmol/L,低危组血清HMGA1、CXCL16水平分别为(122.54±17.78)ng/mL、(4.45±1.32)pmol/L,均显著高于对照组[(106.32±15.54)ng/mL、(2.45±0.03)pmol/L],且高危组均明显高于低危组,差异均有统计学意义(P<0.05)。Logistic回归分析结果显示,血清HMGA1、CXCL16水平与宫颈癌患者HPV感染分型具有相关性(OR=1.104,95%CI:1.028~1.186;OR=2.911,95%CI:1.605~5.278;P<0.05)。结论宫颈癌患者血清HMGA1、CXCL16水平呈高表达,且高危组患者的血清HMGA1、CXCL16水平较低危组升高明显,血清HMGA1、CXCL16水平与宫颈癌患者HPV感染分型具有一定相关性。

Objective To explore the correlation of serum high mobility group protein A1(HMGA 1)and CXC subfamily chemokine 16(CXCL16)levels with the classification of human papillomavirus(HPV)infection in patients with cervical cancer.Methods Ninety-one cervical cancer patients who admitted to Department of Gynecology,Second Affiliated Hospital of Guangxi Medical University from July 2020 to July 2021 were retrospectively selected as the study object,HPV infection were tested for subtyping in all patients.According to the examination results,45 patients(HPV infection genotypes,HPV16,18,31,33,35,39,451,51,and 52,56,58,58,59,66,68)were selected as the high-risk group,46 patients(HPV infection classification as HPV6,11,42,43,44)were selected as the low-risk group,another 50 healthy volunteers who came to Second Affiliated Hospital of Guangxi Medical University for physical examination were selected as the control group.The serum HMGA1 level of the three groups was detected by microparticle enzyme immunoassay,and the serum CXCL16 level of the three groups was detected by enzyme-linked immunosorbent assay;Logistic regression analysis was used to investigate the correlation between serum levels of HMGA1 and CXCL16 and HPV infection subtypes(high-risk and low-risk groups)in patients.Results The levels of serum HMGA1 and CXCL16 in the high-risk group were(156.23±16.14)ng/mL and(10.45±3.12)pmol/L,respectively,the levels of serum HMGA1 and CXCL16 in the low-risk group were(122.54±17.78)ng/mL and(4.45±1.32)pmol/L,respectively,which were significantly higher than those in the control group[(106.32±15.54)ng/mL and(2.45±0.03)pmol/L],and HMGA1 and CXCL16 in the high-risk group were significantly higher than those in the low-risk group,the differences were statistically significant(P<0.05).There was a correlation between serum levels of HMGA1 and CXCL16 and HPV infection subtypes in patients(OR=1.104,95%CI:1.028-1.186;OR=2.911,95%CI:1.605-5.278;P<0.05)according to the result of Logistic regression analysis.Conclusion The serum levels of HMGA1 and CXCL16 in cervical cancer patients are highly expressed,and the levels of HMGA1 and CXCL16 in the high-risk group are significantly higher than those in the low-risk group.There is a certain correlation between the serum levels of HMGA1 and CXCL16 and HPV infection typing in cervical cancer patients.