壳寡糖及其相关衍生物对幽门螺旋杆菌的抑菌作用

The Antibacterial Effect of Chitosan Oligosaccharide and Its Derivative on Helicobacter pylori

采用打孔方法研究以壳寡糖为代表的益生元对于幽门螺旋杆菌的抑制作用。试验结果显示:不同浓度的壳聚糖、羧甲基壳聚糖未产生抑菌环。壳寡糖B(脱乙酰度为94%)在质量浓度为50 mg/mL的条件下,所形成的抑菌环半径为(4.1±0.23)mm。在质量浓度为10 mg/mL的阳性对照(甲硝唑)条件下,所形成的抑菌环半径为(5.5±0.15)mm。高脱乙酰度的壳寡糖B(50 mg/mL)与幽门螺旋杆菌共培养后导致菌体生物积累量下降,而壳寡糖A(50 mg/mL)对其生长无明显影响。质量浓度为50 mg/mL的高脱乙酰度壳寡糖B具有抑制幽门螺旋杆菌的作用,而壳寡糖A(脱乙酰度为80%)、壳聚糖和羧甲基壳聚糖不具有抑制幽门螺旋杆菌的作用。

We use hole method to study the antibacterial effect of the chitosan and its derivative on Helicobacter pylori,the results shows that it can not generate antibacterial circle in different concentration of chitosan and carboxymethyl chitosan.Chitosan oligosaccharide B(degree of deacet ylation,DD=94%,50 mg/mL).has generated the antibacterial circle of Helicobacter pylori,the radius of bacteriostatic ring is(4.1±0.23)mm,while positive control(metronidazole,10 mg/mL)is(5.5±0.15)mm.Chitosan oligosaccharide B(50 mg/mL)can vividly decrease the biological mass of Helicobacter pylori,while the chitosan oligosaccharide A(50 mg/mL)can not influence the growth of strain.Conclusion shows that chitosan oligosaccharide B(50 mg/mL)has the ability to restrain the Helicobacter pylori.The chitosan oligosaccharide A(deacetylation degree of 80%),chitosan and carboxymethyl chitosan provided in this experiment did not have the effect of inhibiting Helicobacter pylori.