发芽绿豆多酚提取物对Ⅱ型糖尿病小鼠糖脂代谢的改善作用

The Ameliorative Effect of Polyphenol Extract from Sprouting Mung Bean on Glycolipid Metabolism in Type 2 Diabetic Mice

目的:探究发芽绿豆多酚提取物对Ⅱ型糖尿病(T2DM)模型小鼠糖脂代谢的改善作用。方法:根据C57BL/6小鼠体重随机分为正常对照组、模型组、发芽绿豆多酚提取物高、中、低剂量组、阳性对照组。通过高脂饮食联合腹腔注射链脲佐菌素(STZ)的方法 建立2型糖尿病小鼠模型。连续灌胃5周后,测定小鼠空腹血糖值、葡萄糖耐量、脏器指数、血脂水平等指标,观察肝脏组织形态。结果:与模型组相比,发芽绿豆多酚提取物能降低小鼠空腹血糖,并显著提高其糖耐量水平(P<0.01);改善T2DM小鼠血清中血脂指标(TC、TG、LDL-C、HDL-C)的浓度(P<0.01);降低小鼠血清ALT、AST的活力,并对STZ诱导的糖尿病小鼠肝脏组织细胞形态有一定的修复作用。结论:一定剂量的发芽绿豆多酚提取液可以有效改善Ⅱ型糖尿病小鼠高血糖和血脂代谢紊乱的症状。

Objective:To investigate the improvement of glycolipid metabolism in mice with type 2 diabetes mellitus(T2DM)induced by polyphenol extract from mung bean sprout.Methods:C57BL/6 mice were randomly divided into normal control group,model group,high,medium and low dose groups of germinated mung bean polyphenol extract and positive control group.A mouse model of type 2 diabetes was established by high fat diet combined with intraperitoneal injection of Streptozotocin(STZ).After continuous gavage for 5 weeks,fasting blood glucose,glucose tolerance,organ index,blood lipid level and liver morphology were measured.Results:during the experiment,compared with the model group,the polyphenol extract of germinated mung bean could reduce the fasting blood glucose and significantly improve the glucose tolerance of mice(P<0.01);Improve the concentration of serum lipid indexes(TC,TG,LDL-C,HDL-C)in T2DM mice(P<0.01);The activity of ALT and AST in serum of mice was reduced,and the morphology of liver tissue in STZ induced diabetic mice was repaired.Conclusion:a certain dose of germinated mung bean polyphenols extract can effectively improve the symptoms of hyperglycemia and dyslipidemia in type 2 diabetic mice.