驼乳对小鼠急性酒精性肝损伤的干预作用

The Intervention of Camel Milk on Acute Alcoholic Liver Injury in Mice

目的:探讨驼乳缓解小鼠急性酒精性肝损伤的作用机制。方法:将ICR小鼠随机分为正常组(NC)、模型组(ET)、驼乳组(CM)和驼乳干预组(ET+CM),通过测定肝组织(AST和ALT)和血清指标(TNF-α、IL-10、1L-6和IL-1β)来反映驼乳对肝脏的保护作用;基于转录组学探讨驼乳发挥保肝、护肝作用的机理。结果:与ET组相比,驼乳干预降低了酒精诱导的AST、ALT、TNF-α、1L-6和IL-1β水平,增加了IL-10水平。对4组小鼠肝组织进行转录组测序,在ET组和ET+CM组中共检出1 426个差异表达基因(其中673个为上调基因,753个为下调基因)。功能注释和富集分析结果 显示,差异表达基因主要显著富集在MAPK、Toll-like和NF-κB信号3条炎症通路。对CD14、TLR5、TLR9等6个代表性差异基因进行实时荧光定量PCR测定。结果 显示,与模型组相比,驼乳干预后能显著降低CD14、IL-1β、IL-6、TLR5和TLR9表达量,显著升高IL-10的表达量,与转录组测序结果一致,验证了测序数据的准确性。研究结果为解析驼乳对酒精性肝损伤的分子机制奠定了良好的基础。

Objective:To explore the molecular mechanism of camel milk to relieve acute alcoholic liver injury in mice.Methods:ICR mice were randomly divided into normal group(NC),model group(ET),camel milk group(CM)and camel milk intervention group(ET+CM),and measured liver tissue(AST and ALT)and serum indexes(TNF-α,IL-10,1L-6 and IL-1β)to reflect the protective effect of camel milk on the liver;and based on transcriptomics research methods to explore the mechanism of camel milk to protect the liver.Results:Compared with the ET group,camel milk intervention reduced the alcohol-induced AST,ALT,TNF-α,1L-6 and IL-1βlevels,and at the same time increased IL-10 levels.Furthermore,transcriptome sequencing was performed on four groups of mouse liver tissues.The results showed that a total of 1426 differentially expressed genes were detected in the ET group and the ET+CM group(673 were up-regulated genes and 753 were down-regulated genes),and then combined with gene function and enrichment analysis,it was found that the differentially expressed genes were mainly significantly enriched In the three inflammatory pathways of MAPK,Toll-like and NF-κB signaling.Six representative differential genes,including CD14,TLR5 and TLR9,were determined by real-time fluorescence quantitative PCR.The results showed that compared with the model group,the expression levels of CD14,IL-1β,IL-6,TLR5 and TLR9 were significantly decreased after camel milk intervention,and the expression levels of IL-10 were significantly increased,which was consistent with the transcriptomic sequencing results,and verified the accuracy of sequencing data.The results laid a good foundation for the analysis of the molecular mechanism of camel milk on alcoholic liver injury.