穴位埋线对心肌梗死大鼠巨噬细胞MerTK胞葬作用的影响

Effect of Acupoint Catgut Embedding on MerTK Cell Burial of Macrophages in Rats with Myocardial Infarction

目的观察“内关”和“心俞”穴位埋线对心肌梗死大鼠巨噬细胞Mer受体酪氨酸激酶(MerTK)相关表达影响,探讨“内关”和“心俞”穴位埋线对心肌梗死大鼠心肌肥大其可能的机制作用。方法雄性大鼠(SD)采用左冠状动脉前降结扎法构建心肌梗死模型,按照随机数字法分为伪手术组、模型组、“内关”和“心俞”穴位埋线组、培哚普利组,每组5只,其中伪手术组只开胸撕开心包膜。术后第2天开始干预治疗,以第7、28天为观测点。苏木精-伊红(HE)染色观察心肌组织病理形态,酶联免疫吸附试验(ELISA)检测血清IL-6、TNF-α、IL-1β浓度水平,蛋白质印迹(Western blotting)检测心肌组织MerTK、p-Mertk蛋白表达。结果(1)与伪手术组大鼠相比较,模型建构后7、28 d,模型组大鼠HE染色心肌细胞形态异常、错乱排列,游离细胞核增多,见明显的细胞间质且心肌细胞横截面积增大(P<0.05),血清IL-6、TNF-α、IL-1β浓度呈明显上升趋势,MerTK蛋白表达降低(P<0.05),p-MerTK蛋白表达升高(P<0.05)。(2)与模型组大鼠相比较,治疗后7、28 d,两治疗组大鼠HE染色心肌细胞形态和大小相对规则,排列相较平行有序,心肌细胞核游离及肥大的情况均有不同程度改善且心肌细胞横截面积均不同程度降低(P<0.05),IL-6、TNF-α、IL-1β浓度均下降(P<0.05),MerTK蛋白表达均不同程度升高(P<0.05),p-MerTK蛋白表达不同程度降低。结论“内关”和“心俞”埋线能够改善心肌梗死大鼠心肌肥大的程度,其机制可能与维持心肌组织中巨噬细胞表面受体MerTK的表达、降低血清炎症细胞因子含量相关。

Objective To observe the effect of catgut embedding at"Neiguan PC6"and"Xinshu BL15"acupoints on Mer receptor tyrosine kinase(MerTK)related expression of macrophages in rats with myocardial infarction,and to explore the possible mechanism of catgut embedding at"Neiguan PC6"and"Xinshu BL15"acupoints on myocardial hypertrophy.Methods Male rats(SD)were randomly divided into sham,model,"Neiguan PC6"and"Xinshu BL15"acupoint catgut embed,perindopril and 5 rats in each group.Intervention treatment started on the second day after operation,with 7 and 28 days as observation points.Hematoxylin eosin(HE)staining was used to observe the pathological morphology of myocardial tissue,and enzyme-linked immunosorbent assay(ELISA)was used to detect serum IL-6,TNF-α,IL-1βThe expression of MerTK and p-Mertk protein in myocardium was detected by Western blotting.Results(1)Compared with the rats in the sham operation group,7 and 28 days after the model was constructed,the myocardial cells in the model group were abnormal in morphology and disordered arrangement,and the number of free nuclei was increased.There was obvious intercellular substance and the cross-sectional area of myocardial cells was increased(P<0.05).Serum IL-6,TNF-α,IL-1βThe concentration showed a significant upward trend,MerTK protein expression decreased(P<0.05),p-MerTK protein expression increased(P<0.05).(2)Compared with rats in the model group,on the 7th and 28th day after treatment,the shape and size of myocardial cells stained with HE in the two treatment groups were relatively regular,and the arrangement was relatively parallel and orderly.The situation of myocardial cell nuclear dissociation and hypertrophy was improved to varying degrees,and the cross-sectional area of myocardial cells was reduced to varying degrees(P<0.05).IL-6,TNF-α,IL-1βThe concentration decreased(P<0.05),MerTK protein expression increased in varying degrees(P<0.05),and p-MerTK protein expression decreased in varying degrees.Conclusion"Neiguan PC6"and"Xinshu BL15"catgut embedding can improve the degree of myocardial hypertrophy in rats with myocardial infarction.The mechanism may be related to maintaining the expression of macrophage surface receptor MerTK in myocardial tissue and reducing the content of serum inflammatory cytokines.