摘要
The surface topography of implants plays a major role in osteogenesis and immunomodulation.In this study,three types of TiO_(2) nanoarrays including nanorod arrays with a diameter of 45 nm(TiO_(2)-N),nanorod arrays with a diameter of 60 nm(TiO_(2)-N N),and nanocone arrays(TiO_(2)-NW)are prepared on titanium and the behavior of bone marrow stromal cells(BMSCs)and polarization of macrophages are studied.Compared to the planar titanium control,TiO_(2) nanoarrays facilitate osteogenesis of BMSCs and stimulate the pro-healing M2 phenotype.However,adhesion,spreading,proliferation,and osteogenic differentiation of BMSCs are more pronounced on TiO_(2)-N N than both TiO_(2)-N and TiO_(2)-NW.TiO_(2)-NN also produces the best immune microenvironment,while TiO_(2)-NW is more favorable than TiO_(2)-NN from the viewpoint of cell adhesion and spreading of osteoblasts.
基金
supported by the National Natural Science Foundation of China (No.52171240)
the Postdoctoral Science Foundation of China (No.2021M691992)
the Major Projects in Research and Development of Shanxi (Projects of International Cooperation,No.201803D421090)
the City University of Hong Kong Donation Research Grant (No.DON-RMG 9229021)
the Hong Kong PDFS-RGC Postdoctoral Fellowship Scheme (Nos.PDFS2122-1S08 and CityU 9061014)
the Hong Kong HMRF (Health and Medical Research Fund) (Nos.2120972 and CityU 9211320).
