正交实验优化重楼皂苷Ⅰ的微波提取工艺

Optimization in Microwave Extraction Process of PolyphyllinⅠby Orthogonal Experiment

在单因素实验的基础上,通过正交实验优化重楼皂苷Ⅰ的微波提取工艺。确定最佳提取工艺为:乙醇体积分数45%、微波功率300 W、微波温度80℃、微波时间35 min、料液比1∶15(g∶mL),在此条件下,重楼皂苷Ⅰ提取率达到5.47%。该方法操作简单、省时、高效,可用于重楼皂苷Ⅰ的提取。

We optimized the microwave extraction process of polyphyllinⅠby orthogonal experiments on the basis of single-factor experiments.The optimum extraction conditions are determined as follows:the ethanol volume fraction of 45%,the microwave power of 300 W,the microwave temperature of 80℃,the microwave time of 35 min,and the solid-liquid ratio of 1∶15(g∶mL).Under above conditions,the extraction rate of polyphyllinⅠcan reach 5.47%.This method is simple,time-saving,and efficient,which can be used for the extraction of polyphyllinⅠ.