lncRNA SNHG5和miR-132-3p在子痫前期病人胎盘组织中的表达关系研究

Study on the relationship between lncRNA SNHG5 and miR-132-3p expression in placenta tissue of preeclampsia patients

目的:观察长链非编码RNA小核仁RNA宿主基因5(lncRNA SNHG5)和miR-132-3p在子痫前期(PE)病人胎盘组织中的表达,分析二者的相关性及对PE的诊断价值。方法:选取112例PE病人作为研究对象,根据PE严重程度分为重度组58例和轻度组54例,另外选取同期健康妊娠孕妇60例作为对照组。采用实时荧光定量PCR检测胎盘组织中lncRNA SNHG5和miR-132-3p表达;Pearson相关分析观察PE病人胎盘组织中lncRNA SNHG5与miR-132-3p的相关性,及lncRNA SNHG5、miR-132-3p与临床指标的相关性;ROC曲线分析lncRNA SNHG5和miR-132-3p水平对PE的诊断价值。结果:轻度组和重度组PE病人24 h尿蛋白、收缩压以及舒张压水平均高于对照组(P<0.05);重度组收缩压、舒张压均高于轻度组(P<0.05)。轻度组、重度组lncRNA SNHG5水平低于对照组(P<0.05),miR-132-3p水平高于对照组(P<0.05);重度组lncRNA SNHG5水平低于轻度组(P<0.05)。胎盘组织中lncRNA SNHG5、miR-132-3p表达与PE病人24 h尿蛋白、收缩压和舒张压均有相关关系(P<0.05~P<0.01),与年龄、孕周和体质量指数无明显相关关系(P>0.05)。PE病人胎盘组组织中lncRNA SNHG5与miR-132-3p表达呈负相关关系(r=-0.509,P<0.05)。ROC曲线分析结果显示,胎盘组织中lncRNA SNHG5、miR-132-3p诊断PE的曲线下面积分别为0.865、0.883,截断值分别为0.93、1.44,敏感度分别为87.9%、79.3%,特异性分别为71.7%、83.3%;二者联合诊断PE的曲线下面积为0.921,敏感度和特异性分别为89.7%、81.7%。结论:PE病人胎盘组织中lncRNA SNHG5呈低表达,miR-132-3p呈高表达,且二者呈负相关关系,为PE的诊断和靶向治疗提供了新的思路。

Objective:To observe the expression of long non-coding RNA small nucleolar RNA host gene 5(lncRNA SNHG5)and miR-132-3p in placental tissue of preeclampsia(PE)patients,and to analyze the correlation between them,and their diagnostic value for PE.Methods:A total of 112 patients with PE were selected as the study subjects,and divided into severe group(n=58)and mild group(n=54)according to the severity of PE.Sixty healthy pregnant women in the same period were selected as the control group.Real-time fluorescence quantitative PCR was used to detect the expression of lncRNA SNHG5and miR-132-3p in placental tissue.Pearson correlation analysis was applied to observe the correlation between lncRNA SNHG5 and miR-132-3p in placental tissue of PE patients,and the correlation between lncRNA SNHG5,miR-132-3p and clinical indicators.ROC curve was employed to analyze the diagnostic value of lncRNA SNHG5 and miR-132-3p levels for PE.Results:The level of 24 h urinary protein,systolic blood pressure and diastolic blood pressure of PE patients in the mild group and severe group was higher than that in the control group(P<0.05),and the level of systolic blood pressure and diastolic blood pressure in the severe group was higher than that in the mild group(P<0.05).The level of lncRNA SNHG5 in the mild group and severe group was lower than that in the control group(P<0.05),and the level of miR-132-3p was higher than that in the control group(P<0.05);the level of lncRNA SNHG5 in the severe group was lower than that in the mild group(P<0.05).The expression of lncRNA SNHG5 and miR-132-3p in placental tissue was correlated with 24 h urine protein,systolic blood pressure and diastolic blood pressure of PE patients(P<0.05 to P<0.01),but was not correlated with age,gestational week and body mass index(P>0.05).There was a negative correlation between lncRNA SNHG5 and miR-132-3p expression in placental tissue of PE patients(r=-0.509,P<0.05).The results of ROC curve analysis showed that the area under the curve of lncRNA SNHG5 and miR-132-3p in placental tissue in the diagnosis of PE was 0.865 and 0.883,the cutoff value was 0.93 and 1.44,the sensitivity was 87.9%and 79.3%,and the specificity was 71.7%and 83.3%,respectively;the area under the curve of the combination of lncRNA SNHG5 and miR-132-3p in the diagnosis of PE was 0.921,the sensitivity and specificity were 89.7%and 81.7%,respectively.Conclusions:LncRNA SNHG5 is lowly expressed and miR-132-3p is highly expressed in the placenta tissue of PE patients,and they are negatively correlated,which provides new ideas for the diagnosis and targeted therapy of PE.