基于HPLC指纹图谱分析不同炮制方式对九制白首乌化学成分的影响

Effect of different processing methods on chemical constituents of nine processed Cynanchum auriculatum by HPLC fingerprint

目的:探究并比较在九蒸九晒和九蒸九烘不同干燥工艺过程中,白首乌外观性状、浸出物和化学成分的变化规律。方法:按照《中国药典》(2020年版)方法对白首乌生品及炮制品的醇溶性浸出物进行测定,建立其高效液相色谱(HPLC)指纹图谱分析法。结果:白首乌醇溶性浸出物在2种干燥方式的炮制过程中均呈现先降低后平稳的趋势;共检出32个峰,其中16个共有峰,指认出4-羟基苯乙酮、2,5-二羟基苯乙酮和2,4-二羟基苯乙酮3个化学成分并进行含量测定。结论:白首乌九蒸九烘和九蒸九晒的工艺结果无明显差别,可考虑用九蒸九烘的现代工艺代替传统的九蒸九晒工艺。

OBJECTIVE To explore and compare the changes of appearance,extract and chemical composition of Cynanchum auriculatum in different drying processes of nine sun-drying and nine oven-drying.METHODS According to Chinese Pharmacopoeia(2020 Edition)method,the alcohol soluble extracts of raw and processed products of Cynanchum auriculatum were determined,and the HPLC fingerprint analysis method was established.RESULTS During the processing of the two drying methods,the alcohol soluble extract of Cynanchum auriculatum showed a trend of decreasing first and then stabilizing.Thirty-two peaks were detected in the HPLC fingerprints,including sixteen common peaks,and three chemical components(4-hydroxyacetophenone,2,5-dihydroxyacetophenone and 2,4-dihydroxyacetophenone)were identified and determined.CONCLUSION There was no significant difference between the two different drying processes,and the modern nine oven-drying process can be considered to replace the traditional nine sun-drying process.