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脂肪酸去饱和酶FAD3蛋白特征及其在大豆中的遗传演化分析 被引量:1

Protein Feature and Genetic Evolution Analysis of Fatty Acid Desaturase FAD3 in Soybean
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摘要 微体ω-3脂肪酸去饱和酶(Fatty Acid Desaturase 3,FAD3)是植物种子α-亚麻酸生物合成的关键酶。尽管很多FAD3基因被分离和鉴定,植物FAD3蛋白结构特征及遗传演化研究较少。为了探究脂肪酸去饱和酶FAD3蛋白特征及其在大豆中的遗传演化情况,本研究分析了37个物种(包括33种植物、2种蓝藻和2种真菌)的51个FAD3蛋白的序列特征和进化关系,并分析了大豆3个GmFAD3(GmFAD3A、GmFAD3B和GmFAD3C)基因在598份材料中的单倍型及其编码蛋白构象。结果发现,植物FAD3蛋白序列和结构较为保守,其进化关系与植物分化进程一致。GmFAD3A、GmFAD3B和GmFAD3C分别包含14,4和4种单倍型。GmFAD3A-1(Hap1/2)在各大豆种植区域均有分布,Hap3/4/5除华南和西北地区外,其他地区均有分布。虽然GmFAD3A-1(Hap1/2/4)和GmFAD3A-1(Hap3/5)编码蛋白构象差异较大,但可能与地理分布并无关联。而GmFAD3A-2(Hap1~5)编码蛋白构象基本相同,表明GmFAD3A-2可能并未受到选择。GmFAD3B(Hap1/2/4)中地方品种占比较高,其中Hap1/2在我国各大豆主要产区均有分布,而Hap3中改良品种占比较高(78.57%),仅分布在东北、华北和华东地区,表明GmFAD3B(Hap1/2/4)和GmFAD3B(Hap3)编码蛋白构象差异可能受地理因素影响的同时受到人工选择。GmFAD3C中Hap1占比极高(98.66%),在我国各大豆种植区域均有分布,说明该基因比较保守,可能在大豆脂肪酸去饱和过程中起到非常重要的作用。本研究为GmFAD3在大豆α-亚麻酸积累中的作用机制解析和高α-亚麻酸育种提供参考。 Microsomal w-3 fatty acid desaturase(Fatty Acid Desaturase 3,FAD3)is a key enzyme in the biosynthesis ofα-linolenic acid in plant seeds.Although many FAD3 genes have been isolated and identified,few studies have been performed on the protein structure and genetic evolution of FAD3 proteins.In order to explore the protein character of fatty acid desaturase FAD3 and its genetic evolution in soybean,a total of 51 FAD3 proteins from 37 species(including 33 plants,2 cyanobacteria and 2 fungi)were studied.Furtherly,14,4,and 4 haplotypes were inferred from 598 soybean germplasms for GmFAD3(GmFAD3A,GmFAD3B,and GmFAD3C).Results showed that the sequence and structure of plant FAD3 proteins were relatively conserved,and their evolutionary relationship was consistent with the process of plant differentiation.GmFAD3A-1(Hapl/2)was distributed in all soybean ecological regions,and Hap3/4/5 was distributed in all regions except South China and Northwest China.Although protein conformations of GmFAD3A-1(Hapl/2/4)and GmFAD3A-1(Hap3/5)were quite different,it may not be related to geographical distribution.And the conformation of Hapl to Hap5 of GmFAD3A-2 was quite similar,indicated that GmFAD3A-2 may not be selected artificially.GmFAD3B(Hapl/2/4)showed higher abundance in landrace,among which Hapl/2 was distributed in all ecological regions in our country,while as high as 78.57%of improved cultivars were observed in Hap3,but only distributed in Northeast China,North China and East China,suggested that the conformational differences between GmFAD3B(Hapl/2/4)and GmFAD3B(Hap3)may be affected by geographical factors,and subject to artificial selection as well.For GmFAD3C,Hapl accountted for a proportion of 98.66%of all genotypes,and distributed in all ecological regions,suggested that this gene may be relatively conservative,and might play a very important role in the desaturation of soybean fatty acids.This study provided a reference for the mechanism analysis of GmFAD3 in soybeanα-linolenic acid accumulation and highα-linolenic acid breeding.
作者 李战 王影 于莉莉 陈伊洁 于欢 王俊 邱丽娟 LI Zhan;WANG Ying;YU Lili;CHEN Yijie;YU Huan;WANG Jun;QIU Lijuan(College of Agriculture,Yangtze University/MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River(Coconstruction by Ministry and Province),Jingzhou 434025,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081,China)
出处 《大豆科学》 CAS CSCD 北大核心 2023年第4期396-405,共10页 Soybean Science
基金 中国农业科学院农业科技创新工程(CAAS-ASTIP)。
关键词 GmFAD3 Α-亚麻酸 大豆 蛋白结构 单倍型分析 GmFAD3 α-linolenic acid soybean protein structure haplotype analysis
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