低剂量PFOS暴露干扰睾丸类固醇激素合成的分子机制研究

Study of the molecular mechanism of low-dose PFOS exposure-induced testicular steroidogenesis disturbance

利用蛋白质组学技术分析了0.015,0.15,1.5mg/kg的PFOS暴露2个月后,大鼠睾丸蛋白质组的差异表达谱.结果显示,PFOS暴露后大鼠血清中孕酮和睾酮的水平均显著上升,并且睾丸组织中56种蛋白质的表达水平发生了显著改变.其中,有10种差异表达蛋白与脂肪酸代谢及睾丸类固醇激素合成密切相关(9种蛋白表达上调,1种表达下调).大部分脂肪酸代谢相关蛋白与睾丸类固醇激素合成相关蛋白在统计学和生物学上均具有显著的相关性.低剂量PFOS暴露可通过加速睾丸中的脂肪酸代谢及类固醇激素合成进程,促进孕酮和睾酮等类固醇激素的合成与分泌,提示普通环境PFOS暴露的男性生殖健康风险.

Proteomic technique was employed to analyze the differential expression profile of rat testicular proteome after exposure to 0.015,0.15 and 1.5mg/kg of PFOS for two months.The results showed that the progesterone and testosterone levels in rat serum were significantly increased,and the expressions of 56 proteins in testis tissue were significantly altered after PFOS exposure.Among them,10 differentially expressed proteins(DEPs)were closely related to fatty acid metabolism and testicular steroidogenesis(9proteins were up-regulated and 1was down-regulated).In addition,most fatty acid metabolism-related DEPs were statistically and biologically correlated with steroidogenesis-related DEPs.These results indicate that low-dose PFOS can accelerate fatty acid metabolism and steroidogenic process in rat testis,ultimately stimulating the synthesis of steroid hormones,progesterone and testosterone.This study can provide new clues to the toxicological mechanism of testicular steroidogenesis disturbance induced by low-dose PFOS exposure,and implicate the male reproductive health risk of humans environmentally exposed to PFOS.