超高效液相色谱-串联质谱法同时测定草莓中 咯菌腈和异菌脲残留量

Determination of Fludioxonil and Iprodione Residues in Strawberry by UPLC-MS/M

为了建立一种同时检测草莓中咯菌腈和异菌脲残留量的分析方法,本研究采用乙腈提取草莓样品中的咯菌腈和异菌脲,GCB、PSA净化,超高效液相色谱-串联质谱检测,外标法定量。结果表明,咯菌腈和异菌脲的质量浓度与峰面积间在0.01~1 mg/L范围内呈良好线性关系,咯菌腈在0.01~3.0 mg/kg添加水平下,平均回收率为83%~105%,相对标准偏差为2.0%~12.1%;异菌脲在0.01~10 mg/kg添加水平下,平均回收率为93%~101%,相对标准偏差为4.9%~9.4%,咯菌腈和异菌脲的最低检出浓度均为0.01 mg/kg。该方法简单、灵敏度高、重现性好,适用于草莓样品中同时测定咯菌腈和异菌脲的残留量。

This study aims to develop a new method for residue analysis of fludioxonil and iprodione in strawberry by UPLC-MS/MS.Fludioxonil and iprodione residues in strawberry were extracted with acetonitrile,purified by GCB and PSA,and determined by UPLC-MS/MS.The quantitative analysis was carried out by external standard method.The results showed that fludioxonil and iprodione showed a good linear relationship at the mass concentration range of 0.01-1 mg/L.At the addition level of 0.01-3.0 mg/kg,the average recoveries of fludioxonil in strawberry were 83%-105%,the relative standard deviations were 2.0%-12.1%.At the addition level of 0.01-10 mg/kg,the average recoveries of iprodione in strawberry were 93%-101%,the relative standard deviations were 4.9%-9.4%.The detection limit of fludioxonil and iprodione were 0.01 mg/kg.The method is simple and reliable,sensitive and reproducible,which can be applied for determining fludioxonil and iprodione in strawberry samples.