硫代半乳糖苷通过抑制半乳糖凝集素-8的糖结合活性改善脑出血继发性脑损伤

Thiodigalactoside improves secondary brain injury in intracerebral hemorrhage by inhibiting glucose-binding activity of Galectins-8

目的探讨小鼠脑出血后半乳糖凝集素-8(Galectins-8)对脑出血急性期脑损伤程度的影响。方法采用胶原酶注入法建立小鼠脑出血模型,制作冰冻切片,采用免疫荧光染色观察血肿周围免疫炎症细胞表达情况,干湿质量法检测脑组织含水量,劳克坚牢蓝/焦油紫(LFB/CV)染色观察CH急性期脑组织的血肿体积、脑肿胀程度。结果脑出血后硫代半乳糖苷抑制Galectins-8的糖结合活性,可显著减少血肿周围活化的小胶质细胞及浸润的中性粒细胞数量(Iba1:t=2.904,P=0.0272;MPO:t=3.503,P=0.0128),抑制脑血肿体积扩大(t=2.486,P=0.0486),减轻脑水肿(t=5.369,P=0.0126),从而改善脑出血后的继发性脑损伤。结论脑出血后Galectins-8一定程度上加重了继发性脑损伤的程度,可能成为治疗脑出血新的潜在治疗靶点。

Objective To investigate the effect of Galectins-8 on the extent of brain damage during the acute phase of intracerebral hemorrhage in mice.Methods A mouse intracerebral hemorrhage model was established by collagenase injection method,and frozen sections were made.Immunofluorescence staining was used to observe the expression of immunoinflammatory cells around the hematoma.The water content of brain tissue was detected by wet and dry weight method.Brain tissue water content was measured using the wet and dry weight method.Luxol Fast Blue/Cresyl Violet(LFB/CV)staining was used to observe the hematoma volume of brain tissues and the degree of brain swelling in the acute stage of intracerebral hemorrhage.Results After intracerebral hemorrhage,thiogalactoside inhibited the sugar-binding activity of Galectins-8,significantly reduced the number of activated microglia and infiltrating neutrophils around the hematoma(Iba1:t=2.904,P=0.0272;MPO:t=3.503,P=0.0128),and inhibited the expansion of the volume of cerebral hematoma(t=2.486,P=0.0486),and reduced cerebral edema(t=5.369,P=0.0126),and thus improved secondary brain injury after intracerebral hemorrhage.Conclusion Galectins-8 somewhat exacerbates the extent of secondary brain injury after intracerebral hemorrhage and may be a new potential therapeutic target for the treatment of intracerebral hemorrhage.