HBV携带者外周血单个核细胞miR-138表达与病毒增殖的关系

Relationship between the expression of miR-138 in peripheral blood mononuclear cells of hepatitis B virus proliferation in carriers

目的探讨乙型肝炎病毒(HBV)携带者外周血单个核细胞(PBMC)miR-138表达与HBV增殖的相关性。方法选取2018年1月—2021年1月东营市人民医院HBV携带者144例(HBV携带组)和体检健康者144名(正常对照组)。收集所有研究对象性别、年龄等一般资料和乙型肝炎血清学标志物、肝功能相关项目检测结果。检测所有研究对象PBMC中miR-138相对表达量,检测HBV携带者HBV DNA载量。根据乙型肝炎血清学标志物检测结果将HBV携带者分为乙型肝炎e抗原(HBeAg)阴性组(63例)和HBeAg阳性组(81例)。采用Pearson相关分析评估miR-138相对表达量与HBV DNA载量的相关性。结果HBV携带组PBMC中miR-138相对表达量显著低于正常对照组(P<0.001)。HBeAg阳性组HBV DNA载量显著高于HBeAg阴性组(P<0.001),PBMC中miR-138相对表达量显著低于HBeAg阴性组(P<0.001)。Pearson相关分析结果显示,HBV携带组PBMC miR-138相对表达量与HBV DNA载量呈负相关(r=-0.841,P<0.05)。结论HBV携带者PBMC中miR-138表达下调,且与HBV增殖密切相关。

Objective To investigate the correlation between the expression of miR-138 in peripheral blood mononuclear cells(PBMC)and hepatitis B virus(HBV)proliferation in HBV carriers.Methods A total of 144 HBV carriers(HBV carrier group)and 144 healthy subjects(healthy control group)were enrolled from Dongying People's Hospital from January 2018 to January 2021.The general data,such as sex and age,hepatitis B serological markers and liver function determination results were collected.The relative expression of miR-138 in PBMC was determined,and the HBV DNA load of HBV carriers was determined.According to the results of hepatitis B serological markers,HBV carriers were classified into hepatitis B e antigen(HBeAg)negative group(63 cases)and HBeAg positive group(81 cases).Pearson correlation analysis was used to evaluate the correlation between the relative expression of miR-138 and HBV DNA load.Results The relative expression of miR-138 in PBMC of HBV carrier group was lower than that of healthy control group(P<0.001).The HBV DNA load in HBeAg positive group was higher than that in HBeAg negative group(P<0.001),and the relative expression of miR-138 in PBMC was lower than that in HBeAg negative group(P<0.001).Pearson correlation analysis showed that PBMC miR-138 relative expression was negatively correlated with HBV DNA load in HBV carrier group(r=-0.841,P<0.05).Conclusions The expression of miR-138 in PBMC of HBV carriers is down-regulated,which is related to HBV proliferation.