温经活血巴布剂中挥发油的提取工艺

Extraction Technology of Volatile Oil from Wenjing Huoxue Cataplasm

目的优选温经活血巴布剂中挥发油的超临界CO_(2)萃取工艺条件。方法在单因素考察萃取温度、分离斧I温度、萃取压力、萃取时间对萃取得率、蛇床子素含量和异欧前胡素综合评分的基础上,正交设计优选萃取温度、萃取压力、萃取时间对所提挥发油综合评分的影响。结果单因素实验中,对所提挥发油综合评分影响较大的因素为萃取温度,萃取压力和萃取时间,正交实验结果表明萃取温度、萃取压力对挥发油综合评分有显著影响,优选的最佳工艺为:萃取温度为55℃,萃取压力为30 MPa,萃取时间为2.0 h。结论本实验优选的萃取工艺稳定可行,可用于该挥发油的提取制备。

Objective To optimize the supercritical CO_(2) extraction conditions of volatile oil from Wenjing Huoxue cataplasm.Methods On the basis of single factor investigation on the comprehensive score of extraction yield,osthole content and isoimperatorin,the effects of extraction temperature,pressure and time on the comprehensive score of extracted volatile oil were optimized by orthogonal design.Results In the single factor experiment,the factors that had a great influence on the comprehensive score of the extracted volatile oil were extraction temperature,extraction pressure and extraction time.The orthogonal experiment results showed that the extraction temperature and extraction pressure had a significant influence on the comprehensive score of volatile oil.The optimized extraction process was as follows:extraction temperature at 55℃,extraction pressure as 30 MPa,and extraction time as 2 h.Conclusion The extraction process optimized in this experiment is stable and feasible,which could be used for the extraction and preparation of the volatile oil.