小麦丙氨酸氨基转移酶家族成员鉴定及初步分析

Identification and Preliminary Analysis of Alanine Aminotransferase Family Genes in Wheat

丙氨酸氨基转移酶(AlaAT)是植物氮代谢中的重要转氨酶类。通过生物信息学分析,在小麦中鉴定出73个AlaAT基因,只有15个与其他物种中已报道的AlaAT基因亲缘关系较近,且分为12个AlaAT和3个GGAT基因,分布在1、2和5号染色群组上。亚细胞定位预测分析发现TaAlaATs定位于细胞质、叶绿体和线粒体中,而TaGGATs定位于过氧化物酶体和细胞质中。启动子分析发现含有多个激素、胁迫响应元件及生长发育调控元件。组织表达分析发现,TaAlaATs基因主要在叶和旗叶中表达量较高,并且对缺氮胁迫均有响应。研究对小麦中丙氨酸氨基转移酶家族成员结构特征及相关功能进行了初步解析,可为进一步研究小麦丙氨酸氨基转移酶家族基因的生物学功能奠定基础,并为小麦氮高效育种工作提供理论依据。

Alanine aminotransferase(AlaAT)is an important aminotransferase class in plant nitrogen metabolism.Through bioinformatics analysis,73 Alanine aminotransferases were identified in wheat.Only 15 genes were closely related to the AlaAT gene reported in other species.These 15 genes,including 12 AlaAT genes and 3 GGAT genes,were mapped on 1,2 and 5 chromosome groups.Subcellular localization analysis showed that TaAlaATs were mainly localized in cytoplasm,chloroplasts and mitochondria,while TaGGATs were localized in peroxisome and cytoplasm.Promoter analysis revealed that there were several hormone response elements,stress response elements and growth and development regulation elements were contained in their promoters region.Gene expression analysis showed that all genes were highly expressed in leaves and flag leaves,and was responsive to nitrogen deficiency stress.In this paper,the structural characteristics and related functions of the alanine aminotransferase family members in wheat were preliminarily identified,which could lay a foundation for further research on the biological functions of the alanine aminotransferase family genes in wheat,and would provide a theoretical basis for wheat nitrogen efficient breeding.