rViRAL:几种常见呼吸道病毒感染宿主应答的基因表达交互查询系统

rViRAL:An Interactive Webserver for Gene Expression of Host Response to Common Respiratory Virus Infections

建立操作便捷、用户友好的在线系统可以实现对海量病毒感染宿主应答数据的深度挖掘,促进对病毒与宿主相互作用的深入了解。通过收录整合1483份批量转录组和超30000个细胞的单细胞转录组数据,以R语言为核心,整合多种先进生物信息工具,设计开发用户友好的在线平台rViRAL(Respiratory Virus Response interActive anaLysis)。在rViRAL上,研究人员通过用户友好的交互界面即可实现对感染后宿主应答的系列分析,包括:差异表达分析、诊断性能评价以及单细胞层面上的分子变化等。此外,rViRAL还提供跨多种常见呼吸道病毒感染类型,对感兴趣的基因进行泛感染比较分析的功能。借助rViRAL,生物学家可以无需任何计算编程技能就可以对感兴趣的基因进行假设探索与验证,这将极大地促进呼吸道病毒感染免疫的数据挖掘、科学讨论和治疗及诊断靶点的发现。

The development of an easy⁃to⁃operate and user⁃friendly webserver could facilitate the data⁃mining of the massive data of host responses to respiratory virus infections and better understandings into the virus⁃host interactions.By collecting and integrating 1,483 samples from Bulk RNA⁃seq and over 30,000 cells from single⁃cell RNA⁃seq manually,a user⁃friendly online platform rViRAL(respiratory Virus Response interActive anaLysis)was developed by R and a suite of advanced bioinformatics tools.With rViRAL,researchers can perform a series of analyzes with simple clicks,including differential expression analysis,diagnostic performance evaluation,and molecular changes at the single⁃cell level.In addition,rViRAL provides pan⁃virus comparative analysis of genes of interest across multiple common respiratory virus infections.Biologists can perform advanced bioinformatic analysis to test their hypothesis without any computational programming skills,which will greatly facilitate data mining,scientific discussion,discovery of therapeutic,and diagnostic targets for host responses to respiratory virus infections.