士的宁对Kv4.3钾通道的影响

Effect of Strychnine on Kv4.3 Potassium Channel

目的 探究士的宁对Kv4.3钾通道的影响。方法 于2019年6—10月在大连理工大学以HEK293T细胞为载体,瞬时转染Kv4.3钾通道,形成Kv4.3-HEK293T细胞,一共选取24个Kv4.3-HEK293T细胞,采用全细胞膜片钳技术,加药前记录的数据为对照组,1μmol/L士的宁处理后的数据为观察组,研究士的宁对Kv4.3电流和动力学参数的影响。结果 与对照组相比,1μmol/L士的宁能抑制Kv4.3电流;1μmol/L士的宁能使半数激活电压由(15.41±2.87)mV降到(-2.10±2.08)mV,差异有统计学意义(t=12.101,P<0.001);1μmol/L士的宁能使半数失活电压降低,但差异无统计学意义(P>0.05);1μmol/L士的宁能使失活后恢复时间τ由(38.64±2.10)ms增加到(48.52±2.79)ms,差异有统计学意义(t=6.930,P<0.001)。结论 士的宁作为中枢兴奋药,其作用机制是可以通过影响Kv4.3钾通道的激活和失活后恢复过程来抑制Kv4.3钾电流。

Objective To explore the effect of strychnine on Kv4.3 potassium channel.Methods From June to Octo‐ber 2019,HEK293T cells were used as vectors in Dalian University of Technology to transiently transfect Kv4.3 potas‐sium channels to form Kv4.3-HEK293T cells.A total of 24 Kv4.3-HEK293T cells were selected,and the whole-cell patch clamp technique was used.The data recorded before drug addition was used as the control group,and the data after 1μmol/L strychnine treatment was used as the observation group to study the effect of strychnine on Kv4.3 cur‐rent and kinetic parameters.Results Compared with the control group,1μmol/L strychnine could inhibit the Kv4.3 current;1μmol/L strychnine could reduce the half activation voltage from(15.41±2.87)mV to(-2.10±2.08)mV,and the difference was statistically significant(t=12.101,P<0.001);1μmol/L strychnine could reduce the half inactivation voltage,but there was no statistically significant difference(P>0.05);1μmol/L strychnine could reduce the recovery timeτafter inactivation from(38.64±2.10)ms increased to(48.52±2.79)ms,the difference was statistically significant(t=6.930,P<0.001).Conclusion As a central stimulant,strychnine can inhibit the Kv4.3 potassium current by affect‐ing the activation and recovery process of the Kv4.3 potassium channel.