增味小承气汤对梗阻性黄疸大鼠胃肠道动力的影响

Effect of modified xiaochengqi decoction on the gastrointestinal motility of rats with obstructive jaundice

目的探讨梗阻性黄疸(OJ)大鼠应用增味小承气汤后对肠道动力及血清胃动素、生长抑素质量浓度的影响。方法将SD雄性大鼠随机分为正常组、假手术组、手术建模梗阻性黄疸+中药组(OJ+M组)和手术建模梗阻性黄疸组(OJ组),每组10只。建立模型并进行实验干预,第3、5、7天自尾静脉取血,酶联免疫吸附实验(ELISA)法测定大鼠血清胃动素、生长抑素水平,术后第8天行活性炭肠推进实验,比较肠道动力变化。结果术后第5、7天,OJ+M组血清胃动素水平高于其他三组,组间差异有统计学意义(P<0.05)。胆管结扎术后第3天,OJ+M组大鼠血清生长抑素水平较假手术组高,但比正常组及OJ组低(P<0.05);术后第5、7天,OJ+M组大鼠血清生长抑素水平显著低于其他三组(P<0.05)。正常组肠推进指数0.596±0.008,假手术组为0.470±0.021,OJ+M组为0.520±0.013,OJ组为0.350±0.009,OJ+M组高于假手术组及OJ组,差异有统计学意义(P<0.05)。结论增味小承气汤通过提升血清胃动素水平、降低血清生长抑素水平,增强了胃肠蠕动功能,加快OJ大鼠术后胃肠功能的恢复。

Objective To explore the effects of modified xiaochengqi decoction on gastrointestinal motility and serum levels of motilin and somatostatin levels in rats with obstructive jaundice(OJ).Methods The healthy SD male rats were randomly divided into the control group,sham group,surgical model of OJ+modified xiaochengqi decoction(OJ+M)group and surgical model of OJ group,with 10 rats in each group.Blood samples were collected via the caudal vein on the 3rd,5th and 7th day after modeling and experimental intervention.ELISA assay was employed to determine serum motilin and somatostatin levels,and then intestinal propulsion test was conducted using activated carbon to observe intestinal peristalsis on the 8th day,thereby identifying the effect of modified xiaochengqi decoction on intestinal peristalsis of rats with OJ.Results On the 5th,7th day after bile duct ligation,serum motilin level in OJ+M group was higher than that in sham group and OJ group,and the differences were statistically significant among groups(P<0.05).On the 3rd day after bile duct ligation,serum somatostatin level was higher in OJ+M group than that in sham group,but lower than that in control group and OJ group,with significant differences(P<0.05).The levels of serum somatostatin were increased in all rats on the 5th and 7th day after the operation,yet the serum somatostatin level was obviously lower in OJ+M group than those in other groups(P<0.05).The intestinal propelling index was 0.596±0.009 in control group,0.470±0.021 in sham group,0.520±0.013 in OJ+M group,and 0.350±0.008 in OJ group,while OJ+M group was higher than sham group and OJ group,and the difference was statistically significant(P<0.05).Conclusion Modified xiaochengqi decoction enhances gastrointestinal peristalsis and accelerates the recovery of gastrointestinal function in OJ rats by increasing serum motilin level and reducing serum somatostatin level.