韭蛆肠道细菌分离鉴定及其抗生素敏感性分析

Isolation and Identification of Intestinal Bacteria from Leeks and Analysis of Antibiotic Sensitivity

为探究韭菜迟眼蕈蚊Bradysia odoriphaga(韭蛆)幼虫肠道可培养细菌的菌群结构及抗生素敏感性。本研究对韭蛆3龄幼虫肠道细菌进行分离培养和16S rDNA鉴定后,采用23种抗生素对其分别进行药敏试验。研究结果表明,经分离鉴定后的6种肠道细菌属于氧化微杆菌Microbacterium oxydans HQ202813.1,葡萄球菌Staphylococcus vitulinus MN640907.1,蜡样芽胞杆菌Bacillus cereus CP045606.2,芽胞杆菌Bacillus sp.MG914019.1,沙雷氏菌属菌株Serratia fonticola MH379711.1,沙福芽胞杆菌Bacillus safensis MN704552.1,并分别命名为B.o1、B.o2、B.o3、B.o4、B.o5、B.o6。对6种可培养细菌的抗生素敏感性进行测试,结果表明,B.o1对21种抗生素都敏感,其中对多粘菌素和磷霉素表现为耐药;B.o2对21种抗生素都敏感,其中对乙酰螺旋素、林可霉素表现为耐药;B.o3对19种抗生素敏感,对青霉素、氨苄西林和多粘菌素耐药;B.o4对19种抗生素敏感,对青霉素、氨苄西林、林可霉素、多粘菌素耐药;B.o5对15种抗生素敏感,对青霉素、氨苄西林、庆大霉素、萘替米星、红霉素、阿奇霉素、麦迪霉素和多粘菌素耐药;B.o6对氨苄西林、磷霉素耐药,对其余19种抗生素敏感。综上所述,韭蛆幼虫肠道细菌菌群具有多样性,存在天然耐药的现象,本研究为进一步开展韭蛆幼虫肠道细菌微生物区系和功能细菌的研究提供了基础。

To investigate the structure of intestinal culturable bacteria and antibiotic sensitivity of Bradysia odoriphaga larvae the intestinal bacteria of the third-instar B.odoriphaga larvae were isolated and cultured and identified by 16S rDNA,and were tested for drug sensitivity with 23 kinds of antibiotics.The results showed that the six types of intestinal bacteria isolated and identified belonged to Microbacilli Oxydans HQ202813.1,Staphylococcus vitulinus MN640907.1,Bacillus cereus CP045606.2,Bacillus sp.Mg914019.1,Serratia fonticola MH379711.1,Bacillus safensis MN704552.1 was named as B.o1,B.o2,B.o3,B.o4,B.o5 and B.o6.The antibiotic sensitivity test of six culturable bacteria showed that B.o1 was sensitive to 21 kinds of antibiotics,among which,it was resistant to polymyxin and fosfomycin.B.o2 was sensitive to 21 antibiotics,and showed resistance to acetylspirin and lincomycin.B.o3 was sensitive to 19 antibiotics and resistant to penicillin,ampicillin and polymyxin.B.o4 was sensitive to 19 antibiotics and resistant to penicillin,ampicillin,lincomycin and polymyxin.B.o5 was sensitive to 15 antibiotics and resistant to penicillin,ampicillin,gentamicin,naphthimidine,erythromycin,azithromycin,medemycin and polymyxin.B.o6 was resistant to ampicillin and fosfomycin,and sensitive to the other 19 antibiotics.To sum up,the intestinal bacterial flora of leek maggot larvae is diverse and has the phenomenon of natural drug resistance.This study provides a basis for further research on intestinal microbial flora and functional bacteria of B.odoriphaga larvae.