肺部活体显微成像技术的研究进展

Research progress of intravital microscopy of the pulmonary immune environment

活体显微镜成像技术的应用开发使研究者得以在细胞水平上实时、原位地观察肺内微循环,对于肺部疾病免疫微环境的研究发挥了重要作用。本文简要介绍了小鼠肺部成像视窗构建技术的研究进展,归纳了目前用于肺活体研究的光学显微镜技术的功能及应用特点,包括宽场荧光显微镜、激光共聚焦显微镜以及双光子扫描显微镜,及其在小鼠肺部炎症及感染模型中的应用,同时指出该技术目前的局限性,为研究者系统性学习和了解该技术提供参考。

The development of intravital microscopy(IVM)has enabled researchers to perform in-situ,real-time observations of pulmonary micro-circulation at the cellular level,and has become instrumental for researching the immune micro-environment of pulmonary diseases.This article introduces the developments in constructing the pulmonary imaging window and summarizes the current light microscopy techniques used for lung IVM with regard to its relevant functional and application features,which includes wide field fluorescence microscopy,confocal microscopy,as well as two-photon microscopy.It then provides examples of IVM of pulmonary immune response in inflammation and infection in murine models,and finally specifies the technological limitations to provide reference for researchers to systematically learn and understand the technology.